An efficient method to produce cordycepin by solid culture using Cordyceps militaris was investigated in this study. Firstly, the changes of cordycepin during various growing periods of solid culture using 5 strains of C. militaris were detected, the best strain and optimal growing period for cordycepin production were determined. Then, by experiments of quadratic rotation-orthogonal combination design and orthogonal design, the medium composition and growth conditions for high yield of cordycepin were optimized. With the optimized method to produce cordycepin, the content of cordycepin in the medium was increased to 0.60%, which was nearly 2 times higher than the highest yield reported.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Cancer immunotherapy using engineered cytotoxic effector cells has demonstrated significant potential. The limited spatial complexity of existing models, however, poses a challenge to mechanistic studies attempting to approve existing approaches of effector cell-mediated cytotoxicity within a three-dimensional, solid tumor-like environment. To gain additional experimental control, we developed an approach for constructing three-dimensional (3D) culture models using smart polymers that form temperature responsive hydrogels.
View Article and Find Full Text PDFAn In Vitro Nutritional Evaluation of Mixed Silages of Drought-Impaired Grass and Sugar Beet Pulp With or Without Silage Inoculants.
J Anim Physiol Anim Nutr (Berl)
January 2025
Centre for Animal Nutrition and Welfare, University of Veterinary Medicine, Vienna, Austria.
Increasing droughts adversely affect grasslands, diminishing the availability and quality of forages for ruminants. We have recently shown that mixed ensiling of drought-impaired grass (DIG) with sugar beet pulp (SBP) improved the conservation and feed value of silage. The application of silage additives may further improve the ruminal degradability, which may thereby shape the fermentation and microbiome in the rumen when those silages are tested as part of dairy diets.
View Article and Find Full Text PDFReclaiming Indigenous systems of healing: experiences of disabled Māori of Māori-centric health service responses in Aotearoa New Zealand during the COVID-19 pandemic.
BMC Health Serv Res
January 2025
School of Nursing, Midwifery, and Health Practice, Wellington Faculty of Health, Victoria University of Wellington, Wellington, New Zealand.
Background: The impact of the pandemic on Indigenous and disabled people's access to healthcare has resulted in significant disruptions and has exacerbated longstanding inequitable healthcare service delivery. Research within Aotearoa New Zealand has demonstrated that there has been success in the provision of healthcare by Māori for their community; however, the experiences of tāngata whaikaha Māori, disabled Māori, have yet to be considered by researchers.
Methods: Underpinned by an empowerment theory and Kaupapa Māori methodology, this research explores the lived realities of tāngata whaikaha Māori or their primary caregivers.
Utility of integrated papyrus-bivalve for bioremediation of aquaculture wastewater.
Environ Sci Pollut Res Int
January 2025
Department of Environmental Management, College of Agricultural and Environmental Sciences, Makerere University, P.O. Box 7062, Kampala, Uganda.
Aquaculture generates substantial amount of residual feeds and faecal matter that accumulate in the culture environment and pollute effluent-receiving water, diminishing its ecological functioning. To devise means of treating nutrient-rich aquaculture wastewater, the efficiency of integrated papyrus-bivalve mesocosms in removing nutrients was evaluated. The mesocosms were fed on water (6600 L) from one brood-stock pond and allowed to settle for 2 weeks.
View Article and Find Full Text PDFEffect of Culture Media on the Yield and Protein Content of (Jacq.) Kumm Mycelia.
Int J Food Sci
December 2024
Department of Biotechnology and Food Technology, Tshwane University of Technology, Pretoria, South Africa.
The development of alternative proteins derived from fungi-based sources is gaining recognition due to their health benefits and lower environmental impact, compared to traditional animal-based sources. In this study, we investigated the culture conditions for mycelia, focusing on the nutritional requirements and yield optimization using solid surface culture and liquid-state culture methods. Our findings indicate that optimal culture conditions involve glucose as the primary carbon source, with an initial pH of 6.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!