AI Article Synopsis
- Unipolar brush cells (UBCs) are a type of interneuron in the cerebellum that can be categorized into three distinct subsets based on the proteins they express.
- In this study, phospholipase C (PLC) beta4 is identified as a novel marker for one of these subsets, which does not overlap with calcium-binding protein calretinin.
- The expression of PLCbeta4 develops exclusively after birth, appearing around postnatal day 12 and reaching maturity at about three weeks.
Article Abstract
Unipolar brush cells (UBCs) are glutamatergic cerebellar interneurons of the granular layer. Previous studies have shown that there are two distinct subsets of UBCs present in the mice cerebellar cortex: calcium-binding protein calretinin (CR) positive and metabotropic glutamate receptor (mGluR)1alpha positive. In this study, we identify phospholipase C (PLC) beta4 as an antigenic marker of a novel subset of UBCs. Double immunolabeling reveals that none of the CR+ subset expresses PLCbeta4. In contrast, most members of the mGluR1alpha subset also express PLCbeta4. In addition, 65% of the PLCbeta4+ subset does not express mGluR1alpha. Thus, there are three distinct UBC subsets in the mouse cerebellum: CR+/PLCbeta4-/mGluR1alpha-, PLCbeta4+/mGluR1alpha-/CR-, and mGluR1alpha+/PLCbeta4+/CR-. Each has a different topographical distribution, both between lobules and mediolaterally within the vermis. The development of PLCbeta4 expression in UBCs is exclusively postnatal--first seen only at P12 and mature at about 3 weeks. A distinct subset of PLCbeta4+ UBCs is also present in primary cerebellar cultures.
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| http://dx.doi.org/10.1007/s12311-009-0092-x | DOI Listing |
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