AI Article Synopsis
- The study aimed to create a High-Performance Liquid Chromatography (HPLC) method for measuring epigoitrin levels in Radix Isatidis, contributing valuable data for its quality control.
- Samples were analyzed using a specific column and mobile phase, achieving a detection wavelength of 245 nm, with a set flow rate and temperature for optimal results.
- Results showed a highly linear response for epigoitrin with an average recovery rate of 98.99%, confirming that the method is accurate and reliable for evaluating the quality of Radix Isatidis.
Article Abstract
Objective: To establish an HPLC method for the content determination of epigoitrin in Radix Isatidis and its preparation, and to provide valuable data for quality control of Radix Isatidis and its preparation.
Method: The samples were separated on a ZORBAX SB-C18 (4. 6 mm x 150 mm, 5 microm) column with the mobile phase of acetonitrile-water-phosphoric acid-triethylamine (8.50 : 90.72 : 0.73 : 0.05) in the flow rate of 0.7 mL x min(-1). The detection wavelength was set at 245 nm. Column temperature was 30 degrees C.
Result: The linear range of epigoitrin was 0.0204-0.3060 microg (r = 0.9998), and the average recovery was 98.99% with the RSD was 1.31% (n = 9).
Conclusion: The method for quantitation of epigoitrin in Radix Isatidis and its preparation was accurate and reliable, which can be used to evaluate the quality of Radix Isatidis and its preparation.
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