AI Article Synopsis
- Intracellular signaling in eukaryotic cells involves various phosphorylated forms of phosphatidylinositol (PtdIns), influenced by multiple PI kinases and phosphatases.
- The primary focus of many cell types is on PI 4-kinase isozymes, which have distinct regulatory activities.
- This chapter discusses a radiometric assay technique for measuring PI 4-kinase activity across different conditions, highlighting its importance as the leading method until better nonradioactive alternatives are developed.
Article Abstract
A wide spectrum of intracellular signaling events mediated by up to seven different phosphorylated forms of phosphatidylinositol (PtdIns) occurs in all eukaryotic cells. The activities of multiple, nondegenerate PI kinases and phosphatases control these signaling events. The PI 4-kinase isozymes account for the major PI kinase activity in many different cell types, and the activity of each isozyme is differentially regulated. The ability to measure and distinguish the activity of individual enzymes is therefore important and forms the subject of the methods in this chapter. We describe the use and application of a versatile radiometric assay to measuring PI 4-kinase activity in a variety of biochemical contexts, from purified enzymes to membrane preparations and permeabilized cells. Until a suitable nonradioactive reagent becomes available, this assay is destined to remain the most widely used method.
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| http://dx.doi.org/10.1007/978-1-60327-115-8_19 | DOI Listing |
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