In our screening for translocase I inhibitors, we found the novel nucleoside antibiotic, A-94964 in the culture broth of Streptomyces sp. SANK 60404. The structure of A-94964 was elucidated primarily by various NMR studies, including a 1H-31P HMBC experiment. A-94964 has a unique structure which possesses a nucleoside moiety and an N-acylglucosamine moiety connected via a phosphate.
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http://dx.doi.org/10.1038/ja.2008.72 | DOI Listing |
J Am Chem Soc
February 2021
Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Natural products containing an -dialkylbenzene moiety exhibit a wide variety of bioactivities, including antibacterial, antifungal, antitumor, and antiangiogenic activities. However, the biosynthetic scheme of the -dialkylbenzene moiety remains unclear. In this study, we identified the biosynthetic gene cluster (BGC) of compounds and in sp.
View Article and Find Full Text PDFBiosci Biotechnol Biochem
June 2019
a Department of Biological Science and Technology, Faculty of Industrial Science and Technology , Tokyo University of Science, Tokyo , Japan.
The immunosuppressive activity of myriocin (ISP-1), a lead compound of fingolimod (FTY720), is derived from its 2-amino-1,3-propandiol structure. A non-proteinogenic amino acid, (2S,6R)-diamino-(5R,7)-dihydroxy-heptanoic acid (DADH), that contains this structure, was recently identified as a biosynthetic intermediate of a dipeptide secondary metabolite, vazabitide A, in Streptmyces sp. SANK 60404; however its effect on adaptive immunity has not yet been examined.
View Article and Find Full Text PDFOrg Biomol Chem
January 2019
Biotechnology Research Center and Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8567, Japan.
The natural product A-94964 is a uridine-derived nucleoside antibiotic isolated from Streptomyces sp. SANK 60404. In this study, we propose a biosynthetic pathway for A-94964 using gene deletion experiments coupled with in silico analysis of the biosynthetic gene cluster.
View Article and Find Full Text PDFBioorg Med Chem Lett
January 2019
Daiichi Sankyo RD Novare Co., Ltd., 1-16-13, Kitakasai, Edogawa-ku, Tokyo 134-8630, Japan. Electronic address:
We herein report a practical one-step glucuronidation method by biotransformation using Streptomyces sp. SANK 60895. This novel direct method of biotransformation has been shown to be more practical and scalable for glucuronidation than previously reported chemical and enzymatic procedures given its simplicity, high β-selectivity, cost-effectiveness, and reproducibility.
View Article and Find Full Text PDFNat Chem Biol
November 2016
Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
Amino-group carrier proteins (AmCPs) mediate the biosynthesis of lysine and arginine in some bacteria and archaea. Here we demonstrate that an uncharacterized AmCP-mediated biosynthetic system functions to biosynthesize the previously uncharacterized and nonproteinogenic amino acid (2S,6R)-diamino-(5R,7)-dihydroxy-heptanoic acid (DADH) in Streptomyces sp. SANK 60404.
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