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Auto-excision of selectable marker genes from transgenic tobacco via a stress inducible FLP/FRT site-specific recombination system. | LitMetric

Auto-excision of selectable marker genes from transgenic tobacco via a stress inducible FLP/FRT site-specific recombination system.

Transgenic Res

Biosafety Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, South Korea.

Published: June 2009

AI Article Synopsis

  • - Antibiotic resistance marker genes are helpful in the early stages of plant transformation but may be unwanted afterward, prompting the need for their removal.
  • - The study successfully used an oxidative stress-inducible FLP gene to excise these resistance genes from transgenic plants, specifically in tobacco.
  • - About 13-41% of the regenerated tobacco shoots were found to be free of the unwanted antibiotic resistance markers, demonstrating the effectiveness of this FLP/FRT system for creating marker-free transgenic plants.

Article Abstract

Antibiotic resistance marker genes are powerful selection tools for use in plant transformation processes. However, once transformation is accomplished, the presence of these resistance genes is no longer necessary and can even be undesirable. We herein describe the successful excision of antibiotic resistance genes from transgenic plants via the use of an oxidative stress-inducible FLP gene. FLP encodes a recombinase that can eliminate FLP and hpt selection genes flanked by two FRT sites. During a transformation procedure in tobacco, transformants were obtained by selection on hygromycin media. Regenerants of the initial transformants were screened for selective marker excision in hydrogen peroxide supplemented media and both the FLP and hpt genes were found to have been eliminated. About 13-41% of regenerated shoots on hydrogen peroxide media were marker-free. This auto-excision system, mediated by the oxidative stress-inducible FLP/FRT system to eliminate a selectable marker gene can be very readily adopted and used to efficiently generate marker-free transgenic plants.

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Source
http://dx.doi.org/10.1007/s11248-008-9236-xDOI Listing

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