AI Article Synopsis
- A high-performance capillary electrophoresis method was developed to create fingerprints of Citrus aurantium for quality control.
- The method utilized a specific electrolyte solution, applied a voltage of 16 kV, and successfully extracted 12 distinct peaks from the samples.
- The results showed that the fingerprints from different locations were highly similar, indicating that this method is reliable for ensuring the quality of Citrus aurantium.
Article Abstract
Objective: To develop a high performance capillary electrophoresis method for Citrus aurantium fingerprints to control its quality.
Method: The background electrolyte (BGE) was an 80 mmol x L(-1) boric acid solution containing 15 mmol x L(-1) borate. The pH of the BGE was adjusted to 9.70 with KOH solution. The detection wavelength was 201 nm and a voltage of 16 kV was applied. The sample hydrodynamic injection was 0.4 ps with a duration time of 8 sec. C. aurantium was extracted by water and a set of capillary electrophoresis fingerprints (CEFP) containing 12 co-possessing peaks was obtained.
Result: There were good similarities between the standard CEFP and each set of CEFP of C. aurantium collected from eleven different places, and their similarity coefficients were between 0.973 and 0.996.
Conclusion: The CEFP has acceptable precision, reproducibility and stability and can be used for the quality control of C. aurantium.
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