The use of Cyan Fluorescent Proteins, with a distinctive lifetime signature, opens up new alternatives to track and semi-quantify the relative expression of proteins in vivo using a single excitation source and emission channel.
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http://dx.doi.org/10.1039/b815445g | DOI Listing |
J Fluoresc
January 2025
Institute of Chemical Technology, Matunga, Mumbai, India.
This study introduces an innovative approach to high-resolution latent fingerprint detection using carbon quantum dots (CQDs) biosynthesized from spent coffee grounds, enhanced with nitrogen doping. Conventional fingerprinting methods frequently use hazardous chemicals and are costly, highlighting the need for eco-friendly, affordable alternatives that preserve detection quality. The biosynthesized nitrogen-doped CQDs exhibit strong photoluminescence and high stability, offering a sustainable, effective alternative for fingerprint imaging.
View Article and Find Full Text PDFAnal Sci
December 2024
School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo, 192-0982, Japan.
A biosensor for biochemical oxygen demand (BOD) was developed based on intracellular 5'-adenosine triphosphate (ATP) measurements in Saccharomyces cerevisiae. Intracellular ATP was measured using an engineered protein named ATeam, comprising a bacterial FF-ATP synthase ε subunit sandwiched between cyan fluorescent protein and mVenus, a modified yellow fluorescent protein. Because the binding of ATP to ATeam induces changes in the fluorescence spectra owing to Fӧrster resonance energy transfer, S.
View Article and Find Full Text PDFLuminescence
December 2024
Department of Chemistry, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, India.
Many industries use copper metal ions (Cu ions), and their salts are utilized as supplemental materials in both agriculture and medicine. Identifying and monitoring these Cu ions in biological and environmental specimens is crucial due to their association with several health issues. In this investigation, we have designed a simple quinoline-based receptor (E)-3-(((2,4-di-tert-butyl-5-hydroxyphenyl)imino)methyl)-6-methoxyquinolin-2(1H)-one (QAP) containing imine functional groups to inspect its capability to identify metal ions in a semi-aqueous medium.
View Article and Find Full Text PDFNat Commun
December 2024
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130012, P. R. China.
Methamphetamine (MA), as one kind of overflowed synthetic illicit drugs, has posed severe threats to health and social security. However, the on-site and visible fluorescence detection to MA remains limited. Herein, through covalently coupling diphenylacridine (DPA) and dimethylacridine (DMA) with pyridine, two ratiometric fluorescence probes (PyDPA and PyDMA) are constructed, which present rapid response, bathochromic-shifts over 100 nm and visible fluorescence color changes from blue to cyan upon the exposure to methylphenethylamine (MPEA, a simulant of MA).
View Article and Find Full Text PDFAnal Chim Acta
January 2025
Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, School of Chemistry, South China Normal University, Guangzhou, Guangdong, 510006, China. Electronic address:
Background: Tetracycline (TC) is frequently utilized as additives in animal feed. The misuse can lead to the residue in food and threaten human health.
Results: A ratiometric fluorescence method for tetracycline detection is developed based on Zinc-based metal-organic framework (Zn-MOF) materials.
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