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Topo-optical investigations of AA-amyloid deposits from various species.
Acta Histochem
August 2009
Department of Obstetrics and Gynaecology, Institute of Pathology, Clinic of Gynaecology and Obstetrics, University of Rostock, Südring 81, D-18059 Rostock, Germany.
Polarization optical analysis of the surface structures of various fungi.
Acta Histochem
August 2009
Institute of Pathology, Clinic of Gynaecology and Obstetrics, University of Rostock, D-18059 Rostock, Germany.
Vicinal hydroxyl groups of the sugar compounds sialic acid and 9-O-acyl sialic acid can be visualised for polarization optical analysis on the surface of different fungi using several topo-optical reactions. We investigated the presence of these molecules in cultures of Cryptococcus neoformans (heterogeneous form), Saccharomyces cerevisiae, Candida albicans, Candida glabrata, Candida krusei and Candida tropicalis by topo-optical reactions. Additionally, we examined brain and stomach tissues of patients with infections by C.
View Article and Find Full Text PDFTopo-optical investigations on the surface of bacterial cells during the phagocytosis of Klebsiella pneumoniae in mouse.
Acta Histochem
August 2009
Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Szigeti u.12., H-7624 Pécs, Hungary.
Polarisation optical methods provide the means to perform sub-microscopic investigations on structures containing spatially highly ordered molecules, for example the cell envelope of prokaryotic cells. Such structures can evoke birefringence, which can be enhanced or modified by different dyes or reagents, thus providing the possibility of a more specific investigation of the composition and structure of bacterial surface compounds. Klebsiella pneumoniae synthesises sterically different carbohydrate-rich structures, including those of the outermost capsular polysaccharide, the polysaccharide somatic antigen of the lipopolysaccharide molecule and the peptidoglycan layer of the cell wall.
View Article and Find Full Text PDFGeorge Romhányi (1905-1991): pioneer in amyloid research and polarization microscopy, on the 100th anniversary of his birth.
Amyloid
December 2005
Department of Obstetrics and Gynecology, University of Rostock, Rostock, Germany.
George Romhányi was an outstanding pathologist, university teacher and a renowned scientist of the 20th century. After studying medicine and pathology, his scientific interest focused on haemochromogenic reactions and submicroscopic structure research. He was among the first to describe the fibrillar-micellar structure of amyloid, as well as the helical-fibrillar structure of elastic fibres.
View Article and Find Full Text PDFAn improved microscopic slide preparation for staining of tissue-isolated and in vitro amyloid fibrils is described in detail. Specimens were prepared by slow drying of an amyloid fibril suspension on aminoalkyl-silanised glass slides underneath a coverglass. The purpose of this method is to obtain microscopically visible fibers.
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