AI Article Synopsis
- An alphavirus-based DNA vaccine (pSFV1CS-E2) effectively protected pigs from classical swine fever virus (CSFV) through cell-mediated immunity, despite low antibody levels.
- In a mouse model, researchers assessed the vaccine's ability to stimulate immune responses using various assays, including lymphoproliferation and cytokine production.
- The study found that both vaccine versions prompted strong immune responses, with the variant pSFV1CS-E2-UL49 showing even greater enhancement in immune activity due to the addition of pseudorabies virus protein VP22.
Article Abstract
We have previously shown that an alphavirus replicon-vectored DNA vaccine (pSFV1CS-E2) encoding the E2 glycoprotein of classical swine fever virus (CSFV) completely protected the immunized pigs from lethal challenge. These animals developed only low or moderate level viral-specific antibody titers before challenge, implying that cell-mediated immunity (CMI) probably played an important role in the protective immunity against CSFV conferred by the DNA vaccine. In this study, the CMI induced by pSFV1CS-E2 and its derivative pSFV1CS-E2-UL49 encoding a fusion protein of CSFV E2 and pseudorabies virus (PRV) VP22 was evaluated in a mouse model by lymphoproliferation assays based on CFSE or WST-8, intracellular cytokine staining, and cytokine ELISA. The results showed that both vaccines induced CSFV-specific lymphoproliferative responses and cytokine production, and pSFV1CS-E2-UL49 induced stronger lymphoproliferative responses and higher cytokine levels than pSFV1CS-E2. These findings suggest that the alphavirus replicon-delivered DNA vaccines are capable of inducing CMI, and PRV VP22 is able to enhance the immunogenicity of the co-delivered antigen.
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| http://dx.doi.org/10.1016/j.vetimm.2008.12.011 | DOI Listing |
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