Objective: To study the relationship between the condition of cell suspension culture and the polysaccharides content in Achyranthes bidentata.
Method: The methods of orthogonal test and alone factor test were used to study the effect of culture time, inoculation concentration, carbon source and pH.
Result And Conclusion: The results showed that the optimum medium to induce polysaccharides in A. bidentata was 1/4MS + 6-BA 0.5 + 2,4-D 1.0 mg x L(-1) + sucrose 2% + glucose 2% + fructose 1% cultured after 50 d. The suitable pH was 6.0, and the optimum inoculation concentration was 7g x L(-1).
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