AI Article Synopsis
- This chapter presents a method for reliably propagating and culturing renal fibroblasts from renal cortical tissue.
- It details how primary fibroblasts are obtained by growing tissue explants in a culture medium enriched with fetal calf serum.
- The process includes subculturing confluent cells and characterizing them as fibroblasts using specific immunohistochemical and immunocytochemical techniques.
Article Abstract
In this chapter we describe a reliable and reproducible method for the selective propagation and culture of renal fibroblasts derived from explantation of renal cortical tissue in vitro. The chapter outlines how primary renal interstitial fibroblasts are derived from explants grown in medium supplemented with foetal calf serum. The subculture of confluent cells and their ultimate characterisation as fibroblasts through immunohistochemical and immunocytochemical techniques are described in detail.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-59745-352-3_3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Review of the Utility of Established Cell Lines for Isolation and Propagation of the Southern African Territories Serotypes of Foot-and-Mouth Disease Virus.
Viruses
December 2024
Department of Biological Sciences and Biotechnology, School of Life Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye 10071, Botswana.
Cell culture underpins virus isolation and virus neutralisation tests, which are both gold-standard diagnostic methods for foot-and-mouth disease (FMD). Cell culture is also crucial for the propagation of inactivated foot-and-mouth disease virus (FMDV) vaccines. Both primary cells and cell lines are utilised in FMDV isolation and propagation.
View Article and Find Full Text PDFOptimizing In Vitro Propagation of Schönland Using Leaf, Root, and Inflorescence.
Plants (Basel)
January 2025
Instituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, Spain.
, a species native to South Africa, is characterized by its limited growth and scarcity, contributing to high production costs. Countries like China and Turkey are known for exporting globally. Tissue culture offers an efficient method for mass-producing unique and beautiful species such as This study tested Murashige and Skoog (MS) basal media supplemented with various concentrations of IBA (0.
View Article and Find Full Text PDFEx Vivo Propagation of Pinctada Birnavirus Using Mantle Tissue Fragment Culture: Application for Measuring Replication at Different Temperatures, TCID Assay, and UV Sensitivity.
Pathogens
January 2025
Japan Fisheries Research and Education Agency, Pathology Division, Aquaculture Research Department, Fisheries Technology Institute, Minami-Ise 516-0193, Mie, Japan.
Pinctada birnavirus (PiBV) is the causative agent of summer atrophy in pearl oyster ( (Gould)). The disease, which induces mass mortality in juveniles less than 1 year old and abnormalities in adults, was first reported in Japan in 2019. Research on the disease has been hindered by the lack of cell lines capable of propagating PiBV.
View Article and Find Full Text PDFLocal characterization of collagen architecture and mechanical properties of tissue engineered atherosclerotic plaque cap analogs.
Acta Biomater
January 2025
Department of Cardiology, Biomedical Engineering, Cardiovascular Institute, Thorax Center, Erasmus MC, Rotterdam, The Netherlands.
Many cardiovascular events are triggered by fibrous cap rupture of an atherosclerotic plaque in arteries. However, cap rupture, including the impact of the cap's structural components, is poorly understood. To obtain better mechanistic insights in a biologically and mechanically controlled environment, we previously developed a tissue-engineered fibrous cap model.
View Article and Find Full Text PDFMyelin ensheathment and drug responses of oligodendrocytes are modulated by stiffness of artificial axons.
PLoS One
January 2025
Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
Myelination is a key biological process wherein glial cells such as oligodendrocytes wrap myelin around neuronal axons, forming an insulative sheath that accelerates signal propagation down the axon. A major obstacle to understanding myelination is the challenge of visualizing and reproducibly quantifying this inherently three-dimensional process in vitro. To this end, we previously developed artificial axons (AAs), a biocompatible platform consisting of 3D-printed hydrogel-based axon mimics designed to more closely recapitulate the micrometer-scale diameter and sub-kilopascal mechanical stiffness of biological axons.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!