Optimum conditions for the biosynthesis of isomaltulose synthase by submerged cultures of the bacterium Erwinia rhapontici, grown in the presence of 10% sucrose, have been determined (temperature of culturing, 30 degrees C; initial pH level, 7.5; duration of culturing, 54 h). The electrophoretically homogeneous preparation of the enzyme, thus obtained, had a specific activity of 210 U/mg protein. Optimum function of the enzyme was observed at 30 degrees C and pH 6.0. Isomaltulose synthase exhibited maximum stability at 20-30 degrees C and pH 6.0-7.0. The catalytic activity of the enzyme amounted to 3300 U/cm3, which is 40 to 50 times higher that the values reported for strains studied previously.
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