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First Molecular and Phylogenetic Characterization of Equine Herpesvirus-1 (EHV-1) and Equine Herpesvirus-4 (EHV-4) in Morocco.
Animals (Basel)
January 2025
Department of Medicine, Surgery, and Reproduction, Agronomy and Veterinary Institute Hassan II, Rabat 10000, Morocco.
This study aimed to investigate the molecular prevalence and genetic characterization of EHV-1 and EHV-4 in equid populations in Morocco. A total of 154 equids (114 horses, 9 donkeys, and 31 mules) were sampled, with nasal swabs and tissue samples subjected to multiplex real-time PCR for the detection of EHV-1 and EHV-4. Additionally, an isolate from the tissue of an aborted horse fetus was included in the analysis.
View Article and Find Full Text PDFComparison of clinical variables and outcome of 2 natural equine herpesvirus myeloencephalopathy outbreaks induced by equine herpesvirus-1 A2254/N752 strain in sport horses.
J Vet Intern Med
January 2025
Department Animal Medicine and Surgery, Universidad CEU-Cardenal Herrera, CEU Universities, Alfara del Patriarca, Valencia, Spain.
Background: Understanding of equine herpesvirus-1 (EHV-1) myeloencephalopathy (EHM) is complicated by disparities among studies.
Objective: Compare clinical findings and outcome in horses involved in 2 recent EHM outbreaks.
Animals: Twenty-five and 10 horses affected during 2 natural EHM outbreaks were admitted to a veterinary teaching hospital (VTH) in 2021 and 2023, respectively.
Investigation of an Outbreak of Equine Herpesvirus-1 Myeloencephalopathy in a Population of Aged Working Equids.
Viruses
December 2024
Carson Valley Large Animal Clinic, Gardnerville, NV 89460, USA.
The objective of this study was to describe an outbreak of equine herpesvirus-1 myeloencephalopathy (EHM) in a population of aged equids. The outbreak was linked to the introduction of five healthy non-resident horses 15 days prior to the first case of acute recumbency. This fulminant EHM outbreak was predisposed by the grouping of the 33 unvaccinated animals in two large pens with shared water and feed troughs.
View Article and Find Full Text PDFGenome sequence of bovine herpesvirus 5 (BoHV-5) isolate from India.
Microbiol Resour Announc
December 2024
National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, India.
Recently, we identified bovine herpesvirus 5 (BoHV-5) in a vaginal swab from aborted cattle. It was unusual in two aspects: first, its association with abortion (it is otherwise mainly associated with encephalitis), and second, it is the first report from India (as it is mostly restricted to South American countries). In this study, we conducted the genome sequencing of the BoHV-5 isolate and provided insights into its phylogenetic relationships with other BoHV-5 strains.
View Article and Find Full Text PDFComparison of nasal swabs and handmade foam cubes for detecting equine herpesvirus 5 (EHV-5) by quantitative polymerase chain reaction (qPCR).
Can J Vet Res
January 2025
Department of Clinical Sciences (Charbonnel, Lavoie, Leclère), Molecular Diagnostic Laboratory, Centre de diagnostic vétérinaire de l'Université de Montréal (CDVUM) (Grenier St-Sauveur, Gagnon), Swine and Poultry Infectious Diseases Research Centre (CRIPA-FRQNT) (Gagnon), Faculté de Médecine Vétérinaire (Juette), Université de Montréal, 3200 rue Sicotte Saint-Hyacinthe, Québec J2S 2M2; Serge Denis BBA, DVM - Animal Health Consultant Inc. (Denis), Montréal, Québec.
The control of equine respiratory infections is a biosecurity challenge. Respiratory viruses are often rapidly detected using quantitative polymerase chain reaction (qPCR) on nasal swabs. In the past, some laboratories developed handmade techniques to increase the amount of nasal secretions collected, without comparing them with nasal swabs when qPCR replaced the use of viral culture.
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