AI Article Synopsis
- The study explored how changes in bladder gap junctions relate to the micturition reflex in rats with partial bladder-outlet obstruction (BOO).
- 66 female rats were divided into different groups based on the duration of obstruction and relief, and tests were conducted to measure bladder performance and gap-junction protein expression (Cx43).
- Results showed that rats experienced shorter intervals between bladder contractions and increased levels of Cx43 expression during and after BOO, indicating a link between increased gap junction communication and bladder overactivity.
- The findings suggest that while the relief of BOO decreased Cx43 levels, the bladder's overactivity might become permanent despite the temporary changes in gap junctions.
Article Abstract
Introduction: We investigated changes of vesical gap junctions in relation to changes of the micturition reflex in rats with partial bladder-outlet obstruction (BOO).
Materials And Methods: A total of 66 female Sprague-Dawley rats were divided into six groups: sham operation (control); 3, 14, and 28 days after BOO; and 3 and 28 days after relief of BOO lasting for a three-day period. Under urethane anesthesia, isovolumetric cystometry was performed on each group. Expression of mRNA for the gap-junction protein connexin 43 (Cx43) in the bladder was measured in each group. Immunohistochemistry using Cx43 antibody was also performed on the bladder after BOO.
Results: The interval between bladder contractions was shorter in all of the other groups than in the control group. Expression of Cx43 mRNA was increased 3, 14, and 28 days after BOO (the peak increase was twofold), and three days after the relief of BOO, but it returned to the control level by 28 days after relief of BOO. Histologically, smooth muscle hypertrophy was detected in the bladder after BOO and punctate staining of the smooth muscle by Cx43 antibody increased after BOO.
Conclusion: These results suggest that partial BOO produces detrusor overactivity that may depend on increased intercellular communication via gap junctions in the bladder. Relief of BOO led to a decrease of Cx43 mRNA, but detrusor overactivity persisted in the chronic phase, suggesting a reversible change of vesical gap junctions and an irreversible change of bladder activity after BOO.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11255-008-9516-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A truncated isoform of Connexin43 caps actin to organize forward delivery of full-length Connexin43.
J Cell Biol
March 2025
Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah, Salt Lake City, UT, USA.
While membrane proteins such as ion channels continuously turn over and require replacement, the mechanisms of specificity of efficient channel delivery to appropriate membrane subdomains remain poorly understood. GJA1-20k is a truncated Connexin43 (Cx43) isoform arising from translation initiating at an internal start codon within the same parent GJA1 mRNA and is requisite for full-length Cx43 trafficking to cell borders. GJA1-20k does not have a full transmembrane domain, and it is not known how GJA1-20k enables forward delivery of Cx43 hemichannels.
View Article and Find Full Text PDFAmelioration of Gap Junction Dysfunction in a Depression Model by Loganin: Involvement of GSK-3β/β-Catenin Signaling.
J Ethnopharmacol
December 2024
School of Life Sciences & School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, People's Republic of China. Electronic address:
Ethnopharmacological Relevance: Comus officinalis Sieb. et Zucc has significant neuroprotective activity and has been widely studied for its potential to improve cognitive function. Our team's previous research has found that loganin isolated from Comus officinalis has an antidepressant effect.
View Article and Find Full Text PDFAdaptive remodeling of rat adrenomedullary stimulus-secretion coupling in a chronic hypertensive environment.
Cell Mol Life Sci
December 2024
Univ Angers, INSERM, CNRS, MITOVASC, Équipe CARME, SFR ICAT, F-49000 Angers, France.
Chronic elevated blood pressure impinges on the functioning of multiple organs and therefore harms body homeostasis. Elucidating the protective mechanisms whereby the organism copes with sustained or repetitive blood pressure rises is therefore a topical challenge. Here we address this issue in the adrenal medulla, the master neuroendocrine tissue involved in the secretion of catecholamines, influential hormones in blood pressure regulation.
View Article and Find Full Text PDFIn Vivo Neurodynamics Mapping via High-Speed Two-Photon Fluorescence Lifetime Volumetric Projection Microscopy.
Adv Sci (Weinh)
December 2024
State Key Laboratory of Radio Frequency Heterogeneous Integration & Key Laboratory of Optoelectronic Devices and Systems, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, China.
Monitoring the morphological and biochemical information of neurons and glial cells at high temporal resolution in three-dimensional (3D) volumes of in vivo is pivotal for understanding their structure and function, and quantifying the brain microenvironment. Conventional two-photon fluorescence lifetime volumetric imaging speed faces the acquisition speed challenges of slow serial focal tomographic scanning, complex post-processing procedures for lifetime images, and inherent trade-offs among contrast, signal-to-noise ratio, and speed. This study presents a two-photon fluorescence lifetime volumetric projection microscopy using an axially elongated Bessel focus and instant frequency-domain fluorescence lifetime technique, and integrating with a convolutional network to enhance the imaging speed for in vivo neurodynamics mapping.
View Article and Find Full Text PDFTunable Transparent Conductors Based on SnO: Theoretical and Experimental Studies of Codoping.
ACS Omega
December 2024
UCL Institute for Materials Discovery, University College London, Malet Place, London WC1E 7JE, United Kingdom.
Transparent conducting oxides (TCOs) are widely used in modern electronics because they have both high transmittance and good conductivity, which is beneficial for many applications such as light-emitting diodes. Tailoring electronic states and hence the conductive types by design is important for developing new materials with optimal properties for TCOs. SnO, with a wide band gap, low cost, no toxins, and high stability, is a promising host material for TCOs.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!