AI Article Synopsis
- The study investigates how the gene nrt2, which is responsible for nitrate transport in the ectomycorrhizal fungus Hebeloma cylindrosporum, is regulated by both the type of host plant and different sources of carbon.
- It was found that nrt2 expression is undetectable in free-living mycelium with low fructose but is high in mycelium associated with the host plant Pinus pinaster.
- Additionally, while high sugar concentrations can enhance nrt2 expression, excessive ammonium levels can suppress it, indicating that nitrogen levels have a stronger influence on gene expression than sugar levels.
Article Abstract
Although the function of the extramatrical mycelium of ectomycorrhizal fungi is considered essential for the acquisition of nitrogen by forest trees, gene regulation in this fungal compartment is poorly characterized. In this study, the expression of the nitrate transporter gene nrt2 from the ectomycorrhizal basidiomycete Hebeloma cylindrosporum was shown to be regulated by plant host and carbon sources. In the presence of a low fructose concentration, nrt2 expression could not be detected in the free-living mycelium but was high in the extramatrical symbiotic mycelium associated to the host plant Pinus pinaster. In the absence of nitrogen or in the presence of nitrate, high sugar concentrations in the medium were able to enhance nrt2 expression. Nevertheless, in the presence of high fructose concentration, high ammonium concentration still completely repressed nrt2 expression indicating that the nitrogen repression overrides sugar stimulation. This is the first report revealing an effect of host plant and of carbon sources on the expression of a fungal nitrate transporter-encoding gene.
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| http://dx.doi.org/10.1007/s00572-008-0221-2 | DOI Listing |
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