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Characterization of human embryonic stem cells with features of neoplastic progression. | LitMetric

AI Article Synopsis

  • Cultured human embryonic stem (hES) cells can undergo genetic changes that make them similar to cancer cells, leading to potential misinterpretations of their capabilities.
  • Two specific variant hES cell lines, v-hESC-1 and v-hESC-2, show high levels of pluripotency markers without standard chromosomal abnormalities but reveal some cancer-like features such as increased growth and tumor-initiating potential.
  • The study highlights the importance of thoroughly characterizing these cells functionally to differentiate between transformed and normal hES cells, as they may not behave as expected.

Article Abstract

Cultured human embryonic stem (hES) cells can acquire genetic and epigenetic changes that make them vulnerable to transformation. As hES cells with cancer-cell characteristics share properties with normal hES cells, such as self-renewal, teratoma formation and the expression of pluripotency markers, they may be misconstrued as superior hES cells with enhanced 'stemness'. We characterize two variant hES cell lines (v-hESC-1 and v-hESC-2) that express pluripotency markers at high levels and do not harbor chromosomal abnormalities by standard cytogenetic measures. We show that the two lines possess some features of neoplastic progression, including a high proliferative capacity, growth-factor independence, a 9- to 20-fold increase in frequency of tumor-initiating cells, niche independence and aberrant lineage specification, although they are not malignant. Array comparative genomic hybridization reveals an amplification at 20q11.1-11.2 in v-hESC-1 and a deletion at 5q34a-5q34b;5q3 and a mosaic gain of chromosome 12 in v-hESC-2. These results emphasize the need for functional characterization to distinguish partially transformed and normal hES cells.

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Source
http://dx.doi.org/10.1038/nbt.1516DOI Listing

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