Burkholderia pseudomallei is an agent of melioidosis and is closely related to avirulent B. thailandensis. Burkholderia thailandensis has a 15-bp deletion within the variable region of the flagellin gene fliC compared with B. pseudomallei. The difference in the fliC gene might be related to virulence. In the present study, the invasion, internalization and intracellular replication of both phagocytic (mouse macrophage cell line RAW264.7) and non-phagocytic cells (human lung epithelial cell line A549) of B. pseudomallei fliC knockout mutant (MM35) complemented with its own fliC (Cp) or with B. thailandensis fliC (Ct) was compared with those of the wild-type strains of B. pseudomallei (1026b) and B. thailandensis (E257). In phagocytic cells, there was no significant difference in bacterial uptake between Cp and Ct, but MM35 was internalized significantly less compared with 1026b, Cp, Ct and E257. The results suggest that flagella are involved in macrophage invasion. In non-phagocytic cells, Cp and Ct showed similar invasive capacities while 1026b, Cp and Ct showed significantly higher invasiveness than MM35, suggesting that flagella facilitate the non-phagocytic cell invasion. However, the invasive capacity of MM35 was significantly higher than that of E257, suggesting that in addition to the flagella, B. pseudomallei may need other factor(s) to facilitate invasion in non-phagocytic cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/S0035-9203(08)70031-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Human genetic variation reveals FCRL3 is a lymphocyte receptor for .
bioRxiv
December 2024
Department of Molecular Genetics and Microbiology, School of Medicine, Duke University, Durham, NC, USA.
is the gram-negative bacterium responsible for plague, one of the deadliest and most feared diseases in human history. This bacterium is known to infect phagocytic cells, such as dendritic cells and macrophages, but interactions with non-phagocytic cells of the adaptive immune system are frequently overlooked despite the importance they likely hold for human infection. To discover human genetic determinants of infection, we utilized nearly a thousand genetically diverse lymphoblastoid cell lines in a cellular genome-wide association study method called Hi-HOST (High-throughput Human in-vitrO Susceptibility Testing).
View Article and Find Full Text PDFInvades Non-Phagocytic Cells by Clathrin-Dependent Endocytic Pathways and Escapes from Phagocytic Vesicles.
Pathogens
November 2024
College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China.
() is capable of causing pneumonia, arthritis, mastitis, and various other ailments in cattle of all age groups, posing a significant threat to the healthy progression of the worldwide cattle industry. The invasion of non-phagocytic host cells serves as a pivotal mechanism enabling to evade the immune system and penetrate mucosal barriers, thereby promoting its spread. To investigate the differences in invasion into four types of non-phagocytic cells (Madin-Darby bovine kidney (MDBK) cells, embryonic bovine lung (EBL) cells, bovine embryo tracheal (EBTr) cells and bovine turbinate (BT) cells) and further elucidate its invasion mechanism, this study first optimized the experimental methods for invasion into cells.
View Article and Find Full Text PDFRole of Circulating Monocytes and Periodontopathic Bacteria in Pathophysiology of Rheumatoid Arthritis.
Bull Tokyo Dent Coll
December 2024
Department of Internal Medicine, Tokyo Dental College, Ichikawa General Hospital.
Article Synopsis
- Rheumatoid arthritis (RA) is driven by chronic inflammation in the synovial membrane, primarily caused by immune cells like macrophages and osteoclasts that lead to cartilage and bone destruction.
- Circulating monocytes differentiate into macrophages and osteoclasts in RA, with synovial macrophages categorized into proinflammatory (M1) and anti-inflammatory (M2) types.
- Recent studies highlight the link between various subsets of these immune cells and RA, along with a notable association between periodontopathic bacteria and the development of the disease.
Mechanobiology studies of bladder tumor cells using laterally squeezing microfluidic flow cytometry.
Talanta
January 2025
Department of Urology, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin, 300211, China. Electronic address:
The deformability and uptake capability of cells are critical indicators of their biomechanical properties and functional behaviors, particularly in tumor heterogeneity and cancer research. Here, we introduce a microfluidic flow cytometry platform integrated with a laterally adjustable squeezing structure for the characterization of bladder tumor cells (including 5637 and EJ cell lines) and uroepithelial cells (SV-HUC-1 cell line). The deformability of these cell types under varying channel width conditions was clearly assessed using this platform.
View Article and Find Full Text PDFEx Vivo Analysis of the Association of GFP-Expressing and with Human Peripheral Blood-Derived (PBD) Leukocytes over 24 Hours.
Microorganisms
September 2024
Faculty of Science and Engineering, The University of Greenwich at Medway, Central Avenue, Chatham Maritime, Kent ME4 4TB, UK.
Article Synopsis
- Leishmania parasites are spread to mammals (like humans) by sandfly bites and can infect different types of immune cells, but the immune response differs between mice and humans.
- In a study focused on human infection, researchers analyzed immune responses using fresh blood samples and flow cytometry, discovering that neutrophils and monocytes make up the majority of cells responding in the initial hours.
- Interestingly, the study also identified a significant presence of CD4 T cells among the leukocytes, suggesting they might play a role in the early stages of Leishmania infection.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!