AI Article Synopsis
- The study investigates the metabolic processes of Pseudomonas rhodesiae and Pseudomonas fluorescens in water-organic solvent systems, specifically focusing on their growth and bioconversion abilities using terpene substrates.
- Both strains were tested on various terpene substrates; P. rhodesiae was found to effectively produce isonovalal from alpha-pinene oxide, while P. fluorescens displayed versatility by metabolizing limonene through two distinct pathways under different oxygen conditions.
- The research highlights a novel enzymatic isomerization process for converting beta-pinene to alpha-pinene and identifies P. fluorescens as a promising candidate for commercial production of alpha-terpineol, enhancing its potential
Article Abstract
Aims: To study the metabolic profile of Pseudomonas rhodesiae and Pseudomonas fluorescens in water-organic solvent systems using terpene substrates for both growth and biotransformation processes and to determine the aerobic or anaerobic status of these degradation pathways.
Materials And Methods: Substrates from pinene (alpha-pinene, alpha-pinene oxide, beta-pinene, beta-pinene oxide, turpentine) and limonene (limonene, limonene-1,2-oxide, orange peel oil) families were tested. For the bioconversion, the terpene-grown biomass was concentrated and used either as whole cells or as a crude enzymatic extract.
Conclusion: Pseudomonas rhodesiae was the most suitable biocatalyst for the production of isonovalal from alpha-pinene oxide and did not metabolize limonene. Pseudomonas fluorescens was a more versatile micro-organism and metabolized limonene in two different ways. The first (anaerobic, cofactor-independent, noninducible) allowed limonene elimination by synthesizing alpha-terpineol. The second (aerobic, cofactor-dependent) involved limonene-1,2-oxide as an intermediate for energy production through a beta-oxidation process.
Significance And Impact Of The Study: Enzymatic isomerization of beta- to alpha-pinene was described for the first time for both strains. Alpha-terpineol production by P. fluorescens was very efficient and appeared as a promising alternative for the commercial production of this bioflavour.
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| http://dx.doi.org/10.1111/j.1365-2672.2008.03923.x | DOI Listing |
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