Isolation of highly copper-tolerant fungi from the smelter of the Naganobori copper mine, an historic mine in Japan.

Aims: Copper is a critical metal of modern industry, and is the most widespread heavy metal contaminant in wastewater. Therefore, isolation of copper-tolerant microbes having the potential as biosorbent is fascinating not only from an environmental microbiology, but also from a biotechnology view point. In this study, we attempted to isolate highly copper-tolerant microbes from soil samples of the Nabanobori copper mine, the oldest mine in Japan.

Methods And Results: As a result of an enrichment culture, two fungal strains were isolated from soil of the smelter remains. The isolates could grow in a maximum of 200 mmol l(-l) Cu(2+), and grew under a wide pH range. The Cu(2+)-binding capacity of nontreated biomass of the isolates was around 35 mg Cu(2+) g(-1)-biomass. Analysis of 18S rDNA suggested that the isolates belong to the Aspergillus/Penicillium clade, but they represented a distinct lineage against known neighbours.

Conclusion: The isolates were highly copper-tolerant, and their Cu(2+)-binding capacity was comparable to well-studied fungal sorbents. The isolates were implied as novel species. Soil of the historic old mine under weather-beaten conditions might be a suitable source for metal-tolerant microbes.

Significance And Impact Of The Study: The present results advance our understanding of metal-tolerant microbes, and offer a new tool for both environmental control and metal recovery operations.