AI Article Synopsis
- - Calcium ions are crucial for effective neurotransmission, and disturbances in their levels can lead to serious issues in neuronal function.
- - The PMCA (plasma membrane calcium pump) is responsible for maintaining low levels of free calcium in cells, with PMCA2 and 3 being the key isoforms in excitable cells like neurons.
- - Research on PC12 cells (a model for neuroendocrine cells) showed that different neurosteroids influence calcium uptake, and the effect varies based on the specific PMCA isoform present and the structure of the steroids.
Article Abstract
Calcium ions are essential to proper neurotransmission. Impairment in cytosolic Ca(2+) concentration and Ca(2+) signaling disturbs neuronal activity, leading to pathological consequences. In cells, a high-affinity plasma membrane calcium pump (PMCA) keeps free Ca(2+) in the nanomolar range. Among four genes encoding the enzyme, PMCA2 and 3 are primary in excitable cells. To elucidate the relationship between PMCAs' composition and susceptibility for neurosteroid regulation, we obtained PC12 cells with suppressed neuron-specific isoforms and analyzed the effect of selected steroids on Ca(2+) uptake. Our results indicate that hormones affected Ca(2+) transport activity and that this effect depended on both PMCA isoform composition and steroid structure.
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| http://dx.doi.org/10.1196/annals.1410.077 | DOI Listing |
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