The DNA archives developed at veterinary medical teaching hospitals will be important resources for mapping disease loci and identifying underlying genes. The most important feature of a DNA archive is accurate identification or exclusion of diseases in each animal. Such archives will be complimentary resources to tissue banks that are currently available.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2754738PMC
http://dx.doi.org/10.2460/javma.234.1.75DOI Listing

Publication Analysis

Top Keywords

dna archives
8
teaching hospitals
8
development dna
4
archives veterinary
4
veterinary teaching
4
hospitals investigate
4
investigate genetic
4
genetic basis
4
basis disease
4
disease dogs
4

Similar Publications

Background: Traumatic brain injury (TBI) is recognized as one major, potentially modifiable risk factor for neurodegenerative disease (NDD). Autopsy studies describe a range of neuropathologies in a proportion of individuals surviving late after TBI, most frequently the tau associated pathology, chronic traumatic encephalopathy neuropathologic change (CTE-NC). In addition to tau, other NDD pathologies are described.

View Article and Find Full Text PDF

HPV status is an important prognostic factor in oropharyngeal squamous cell carcinoma (OPSCC), with HPV-positive tumors associated with better overall survival. To determine HPV status, we rely on the immunohistochemical investigation for expression of the P16 protein, which must be associated with molecular investigation for the presence of viral DNA. We aim to define a criterion based on image analysis and machine learning to predict HPV status from hematoxylin/eosin stain.

View Article and Find Full Text PDF

Reframing Formalin: A Molecular Opportunity Enabling Historical Epigenomics and Retrospective Gene Expression Studies.

Mol Ecol Resour

January 2025

National Research Collections Australia, Commonwealth Scientific Industrial Research Organisation, Canberra, Australian Capital Territory, Australia.

Formalin preservation of museum specimens has long been considered a barrier to molecular research due to extensive crosslinking and chemical modification. However, recent optimisation of hot alkaline lysis and proteinase K digestion DNA extraction methods have enabled a growing number of studies to overcome these challenges and conduct genome-wide re-sequencing and targeted locus-specific sequencing. The newest, and perhaps most unexpected utility of formalin preservation in archival samples is its ability to preserve in situ DNA-protein interactions at a molecular level.

View Article and Find Full Text PDF

High-sensitivity detection of DNA in tongue swab samples.

J Clin Microbiol

December 2024

Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, USA.

Article Synopsis
  • Tongue swab (TS) sampling combined with quantitative PCR (qPCR) shows promise as an alternative to sputum testing for tuberculosis (TB) diagnosis, although previous studies indicated lower sensitivity.
  • Two strategies were evaluated to enhance sensitivity: one involved centrifugation to concentrate MTB DNA, achieving 83% sensitivity and 100% specificity, while the other utilized sequence-specific magnetic capture, resulting in 90% sensitivity and 97% specificity.
  • These findings suggest that with further development, these methods could lead to more accessible and effective TB testing options.
View Article and Find Full Text PDF

The phyllosphere microbiome can positively or negatively impact plant health and growth, but we currently lack the tools to control microbiome composition. Contributing to a growing collection of bacteriophages (phages) targeting bacteria living in the wheat phyllosphere, we here isolate and sequence eight novel phages targeting common phyllosphere Erwinia and Pseudomonas strains, including two jumbo phages. We characterize genomic, phylogenetic, and morphological traits from these phages and argue for establishing four novel viral genera.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!