RNA interference can be extremely useful in determining the function of an endogenously-expressed protein in its normal cellular environment. In this chapter, we describe a method that uses small interfering RNA (siRNA) to knock down mRNA and protein expression in cultured cells so that the effect of a putative regulatory protein on gene expression can be delineated. Methods of assessing the effectiveness of the siRNA procedure using real time quantitative PCR and Western analysis are also included.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900309 | PMC |
| http://dx.doi.org/10.1007/978-1-60327-575-0_11 | DOI Listing |
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