Self-assembling protein cages provide a wide range of possible applications in nanotechnology. We report the first example of an engineered pH-dependent molecular switch in a virus-like particle. By genetically manipulating the subunit-subunit interface of the E2 subunit of pyruvate dehydrogenase, we introduce pH-responsive assembly into a scaffold that is natively stable at both pH 5.0 and 7.4. The redesigned protein module yields an intact, stable particle at pH 7.4 that dissociates at pH 5.0. This triggered behavior is especially relevant for applications in therapeutic delivery.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/nl8027069 | DOI Listing |
Publication Analysis
Top Keywords
ph-dependent molecular
8
molecular switch
8
design ph-dependent
4
switch caged
4
caged protein
4
protein platform
4
platform self-assembling
4
self-assembling protein
4
protein cages
4
cages provide
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!