Carrot is the most economically important member of the Apiaceae family and a major source of provitamin A carotenoids in the human diet. However, carrot molecular resources are relatively underdeveloped, hampering a number of genetic studies. Here, we report on the synthesis and characterization of a bacterial artificial chromosome (BAC) library of carrot. The library is 17.3-fold redundant and consists of 92,160 clones with an average insert size of 121 kb. To provide an overview of the composition and organization of the carrot nuclear genome we generated and analyzed 2,696 BAC-end sequences (BES) from nearly 2,000 BACs, totaling 1.74 Mb of BES. This analysis revealed that 14% of the BES consists of known repetitive elements, with transposable elements representing more than 80% of this fraction. Eleven novel carrot repetitive elements were identified, covering 8.5% of the BES. Analysis of microsatellites showed a comparably low frequency for these elements in the carrot BES. Comparisons of the translated BES with protein databases indicated that approximately 10% of the carrot genome represents coding sequences. Moreover, among eight dicot species used for comparison purposes, carrot BES had highest homology to protein-coding sequences from tomato. This deep-coverage library will aid carrot breeding and genetics.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00438-008-0411-9 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sustainable microwave-based eutectic mixture assisted purification of low-methoxy pectin and its role as an edible emulsifier in carrot jam preparation.
Food Chem
December 2024
Green Separation Engineering Laboratory, School of Chemical & Biotechnology, SASTRA Deemed to be University, Thanjavur, India. Electronic address:
An optimized microwave-assisted low methoxyl pectin extraction procedure was described. Six task specific deep eutectic solvents (TDES) were used in the extraction of pectin from bilimbi (LMABP) and pomelo peels (LMCGP). Response surface methodology-based optimization of the parameters like feed-to-solvent ratio, extraction time, and microwave power level results in 72.
View Article and Find Full Text PDFSafety of Fezolinetant for Treatment of Moderate to Severe Vasomotor Symptoms Due to Menopause: Pooled Analysis of Three Randomized Phase 3 Studies.
Adv Ther
December 2024
Astellas Pharma Global Development, Northbrook, IL, USA.
Introduction: This study evaluated the safety and tolerability of fezolinetant in women with vasomotor symptoms (VMS) due to menopause in a pooled analysis of data from three 52-week phase 3 studies (SKYLIGHT 1, 2, and 4).
Methods: SKYLIGHT 1 and 2 were double-blind, placebo-controlled studies where women (≥ 40 to ≤ 65 years), with moderate to severe VMS (minimum average ≥ 7 hot flashes/day) were randomized to once-daily placebo, fezolinetant 30 mg or 45 mg. After 12 weeks, those on placebo were re-randomized to fezolinetant 30 mg or 45 mg, while those on fezolinetant continued on their assigned dose for 40 weeks.
Essential oil of (L.) ssp. (Apiaceae) flower: chemical composition, antimicrobial potential, and insecticidal activity on (L.).
Z Naturforsch C J Biosci
January 2025
Laboratory of Molecular Chemistry and Natural Substances, Faculty of Sciences of Meknes, 11201 Zitoune-Meknes B.P, Meknes, Meknes, Morocco.
In order to search for new chemotypes and to carry out a comparative study with the literature, the current study investigated the chemical composition of the essential oil of the flowers of (L.) ssp. using gas chromatography coupled with mass spectrometry (GC-MS).
View Article and Find Full Text PDFPlant Heat Shock Proteins Are More Effective in Enhancing Recombinant Alcohol Dehydrogenase Activity than Bacterial Ones .
Iran J Biotechnol
July 2024
Department of Biotechnology, Sangmyung University, 20 Hongjimun 2-gil, Jongno-gu, Seoul 03016, Korea.
Background: Recombinant proteins produced in the cell factories are used in biological research, pharmaceutical production, and biochemical and agricultural applications. Molecular chaperones, such as heat shock proteins (Hsps), are co-expressed with recombinant proteins to enhance their yield, stability, and activity. When () is used as a cell factory, Hsps are the frequently used co-expression partners.
View Article and Find Full Text PDFCarrot-Derived Rhamnogalacturonan-I Consistently Increases the Microbial Production of Health-Promoting Indole-3-Propionic Acid Ex Vivo.
Metabolites
December 2024
Cryptobiotix SA, 9052 Ghent, Belgium.
Background: Using dietary interventions to steer the metabolic output of the gut microbiota towards specific health-promoting metabolites is often challenging due to interpersonal variation in treatment responses.
Methods: In this study, we combined the ex vivo SIFR (Systemic Intestinal Fermentation Research) technology with untargeted metabolite profiling to investigate the impact of carrot-derived rhamnogalacturonan-I (cRG-I) on ex vivo metabolite production by the gut microbiota of 24 human adults.
Results: The findings reveal that at a dose equivalent to 1.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!