[Construction of tissue-engineering skin with carrier of active composite dermal matrix].

Objective: To construct of tissue engineering skin including active composite dermal matrix.

Methods: The human fibroblasts and bovine collagen with type I were inoculated on the surface of porcine acellular dermal matrix (PADM) for construction of active dermal substitute, then epidermal cells were inoculated on the dermal matrix for gas-liquid interface culture. The tissue-engineering skin was observed by histological examinations.

Results: The structure of fibroblasts in collagen was intact, which was used to construct composite dermal matrix with PADM. The epithelial structure of tissue-engineering skin was similar to that of normal skin with good cell differentiation. Some phenomena were showed in epidermis: basic layer, stratum spinosum, granular layer and stratum corneum, desmosomes.

Conclusion: Fibroblasts-Collagen-PADM can be an optimal dermal matrix for construction of tissue-engineering skin.