Genotyping of benzimidazole susceptible and resistant alleles in different populations of Haemonchus contortus from Himalayan and sub-Himalayan regions of North-West India. | LitMetric
An allele-specific PCR was standardized to diagnose the mutation (Phe to Try) at residue 200 of the isotype 1 beta tubulin gene responsible for benzimidazole resistance in Haemonchus contortus adult and infective larvae. Adult and infective larvae of Haemonchus contortus were collected from sheep under different managemental practices (intensive and extensive) in temperate Himalayan regions (Mukteshwar and Kedarkhatta), sub-himalayan region (Pantnagar) of Uttarakhand state and subtropical region of Uttar Pradesh (Bareilly) in north-west India. Genotyping of adult H. contortus, collected from abomasi of slaughtered sheep reared under extensive management, by AS-PCR revealed that the frequency of resistant (r) alleles was significantly higher (P < 0.001) at Pantnagar (0.57) as compared to Bareilly (0.25) and Mukteshwar (0.08). Also, genotyping of infective larvae of the parasite from intensively managed sheep farms indicated that the frequency of resistant (r) alleles was significantly higher (P < 0.001) at Pantnagar (0.85) as compared to Kedarkattha (0.70) and Bareilly (0.62). The results revealed that managemental practices followed in the areas under study have a direct bearing on the spread of benzimidazole resistant alleles.
Background: is a transmitted respiratory pathogen that causes high morbidity and mortality in children, especially those under 5 years of age. During the implementation of population control measures for COVID-19 in mainland China, the detection rate in pediatric patients decreased. However, with the second wave of the COVID-19 pandemic (2022), the incidence of pneumococcal disease (PD) and even invasive pneumococcal disease (IPD) began to rise again.
Pearl millet {Pennisetum glaucum (L.) R. Br} is a C4 panicoid cereal millet crop grown in arid and semi-arid regions in Africa and Asia for food and fodder.
Escherichia coli producing OXA-48-like carbapenemases (OXA-EC) is considered a high-risk pathogen spread primarily in the community in low- and middle-income countries and nosocomially in high-income countries. We investigated the emergence and spread of OXA-EC in Israel, a high-income country with strong carbapenemase-directed infection control in healthcare institutions, by conducting a population-based study using data and isolates from the national surveillance system. A total of 3,510 incident cases of OXA-EC occurred during 2007-2023.
SAMRC/NHLS/UCT Molecular Mycobacteriology Research Unit, DSI/NRF Centre of Excellence for Biomedical TB Research, Department of Pathology, University of Cape Town, South Africa; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa. Electronic address:
The mycobacterial mutasome - comprising ImuA', ImuB, and DnaE2 - has been implicated in DNA damage-induced mutagenesis in Mycobacterium tuberculosis. ImuB, which is predicted to enable mutasome function via its interaction with the β clamp, is a catalytically inactive Y-family DNA polymerase. Like some other members of the Y-family, ImuB features a recently identified amino acid motif with homology to the RecA N-terminus (RecA-NT).
Infections have imposed strong selection pressures throughout human evolution, making the study of natural selection's effects on immunity genes highly complementary to disease-focused research. This review discusses how ancient DNA studies, which have revolutionized evolutionary genetics, increase our understanding of the evolution of human immunity. These studies have shown that interbreeding between modern humans and Neanderthals or Denisovans has influenced present-day immune responses, particularly to viruses.
