The transcriptional activator beta-catenin is the key mediator of the canonical Wnt signaling pathway. However, beta-catenin does not itself bind DNA, but functions via interaction with T-cell factor (TCF)/ lymphoid-enhancing factor (LEF) transcription factors. These proteins contain a high-mobility group (HMG) box that binds DNA in a sequence-specific manner. Thus, in the case of active Wnt signaling, beta-catenin activates, in cooperation with proteins of the TCF/LEF family, the expression of a wide variety of genes. To date, the list of established Wnt targets is far from complete. The establishment of plasmids harbouring reporter genes under control of the native promoter sequences provides a tool to validate novel putative Wnt targets by directly quantifying the beta-catenin-dependent activation of each specific gene. In this chapter, we describe how to generate such reporter plasmids using the MMP7 promoter as an example.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1007/978-1-59745-249-6_9 | DOI Listing |
Biotechnol Bioeng
January 2025
Department of Biosystems Engineering, Auburn University, Auburn, Alabama, USA.
Bacillus velezensis FZB42 is a prominent plant growth-promoting rhizobacterium and biocontrol agent known for producing a wide array of antimicrobial compounds. The capability to genetically manipulate this strain would facilitate understanding its metabolism and enhancing its sustainable agriculture applications. In this study, we report the first successful implementation of high-efficiency CRISPR-Cas9 genome editing in B.
View Article and Find Full Text PDFJMIR Aging
January 2025
Centre of Expertise in Care Innovation, Department of PXL - Healthcare, PXL University of Applied Sciences and Arts, Hasselt, Belgium.
Background: Advancements in mobile technology have paved the way for innovative interventions aimed at promoting physical activity (PA).
Objective: The main objective of this feasibility study was to assess the feasibility, usability, and acceptability of the More In Action (MIA) app, designed to promote PA among older adults. MIA offers 7 features: personalized tips, PA literacy, guided peer workouts, a community calendar, a personal activity diary, a progression monitor, and a chatbot.
Int J Mol Sci
January 2025
Department of Life Sciences, Ben Gurion University of the Negev, Beer-Sheva 8410501, Israel.
Phenotypic variability in isogenic bacterial populations is a remarkable feature that helps them cope with external stresses, yet it is incompletely understood. This variability can stem from gene expression noise and/or the unequal partitioning of low-copy-number freely diffusing proteins during cell division. Some high-copy-number components are transiently associated with almost immobile large assemblies (hyperstructures) and may be unequally distributed, contributing to bacterial phenotypic variability.
View Article and Find Full Text PDFAppl Environ Microbiol
January 2025
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
Lignin contains a variety of interunit linkages, leading to a range of potential decomposition products that can be used as carbon and energy sources by microbes. β-O-4 linkages are the most common in native lignin, and associated catabolic pathways have been well characterized. However, the fate of the mono-aromatic intermediates that result from β-O-4 dimer cleavage has not been fully elucidated.
View Article and Find Full Text PDFPlant Physiol Biochem
January 2025
Key Laboratory of Saline-alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin, 150040, China; College of Life Science, Northeast Forestry University, Harbin, 150040, China. Electronic address:
The basic helix-loop-helix (bHLH) transcription factors (TFs) play a crucial regulatory role in the growth and development of plants, as well as in their response to environmental stresses. In this study, we identified 94 ChbHLHs from Cerasus humilis, an economically valuable tree native to northern China. We analyzed their evolutionary relationships, gene structures, chromosome distributions, promoter cis-regulatory elements, and collinearity.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!