A quinoline sensitizer-centered lanthanide chelate system of novel design for TR-LRET was prepared; it exhibited high labelling efficiency with a his-tagged protein (ERalpha-LBD) on the Ni-NTA beads, using a mixed metal chelate protocol, and it functioned well in TR-LRET protein-protein interaction assays.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2743944 | PMC |
http://dx.doi.org/10.1039/b814980a | DOI Listing |
Chem Commun (Camb)
January 2009
Department of Chemistry of University of Illinois, 600 South Mathews Avenue, Urbana, IL 61801, USA.
A quinoline sensitizer-centered lanthanide chelate system of novel design for TR-LRET was prepared; it exhibited high labelling efficiency with a his-tagged protein (ERalpha-LBD) on the Ni-NTA beads, using a mixed metal chelate protocol, and it functioned well in TR-LRET protein-protein interaction assays.
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