AI Article Synopsis
- * A novel non-radioactive method was developed to detect LP in industrial CTW samples using nested-PCR and specific probes, which provides a quick and efficient way to determine contamination levels.
- * The study demonstrated that this PCR-ELISA technique was highly specific and successfully identified LP in all tested CTW samples, making it a promising option for routine contamination monitoring.
Article Abstract
Water supply and Cooling Tower Water (CTW) are among the most common sources of Legionella pneumophila (LP) contamination. A nonradio active method is described to detect LP in industrial CTW samples. DNA was purified and amplified by nested -PCR with amplimers specific for the 16s rRNA gene of LP. The 5' end biotinylated oligomer probe was immobilized on sterptavidin B coated microtiter plates. The nested-PCR product was labeled with digoxigenin and then hybridized with 5'-biotinylated probes. The amplification products were detected by using proxidase-labled anti dioxygenin antibody in a colorimetric reaction. The assay detected LP present in 1 L of 5 CTW samples examined. All of the samples were Legionella positive in both culture and PCR-ELISA methods. The PCR-ELISA assay appears to exhibit high specificity and is a more rapid technique in comparison with bacterial culture method. Thus could prove suitable for use in the routine examination of industrial CTW contamination.
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| http://dx.doi.org/10.3923/pjbs.2007.4015.4021 | DOI Listing |
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