AI Article Synopsis
- The study focused on isolating microorganisms that can convert beta-pinene to alpha-pinene, which included 24 different strains from sources like galbanum and soil.
- A bacterial strain was identified as capable of this transformation, which involved specific biotransformation conditions using phosphate buffer and glycerin as a carbon source.
- The optimal conditions for the highest conversion rate were 20 g L(-1) glycerin and 37 degrees Celsius, with the process monitored through gas chromatography.
Article Abstract
The present study describes the isolation of microorganisms capable of producing alpha-pinene from beta-pinene. 24 (15 fungi, 9 bacteria) microorganisms were isolated from galbanum, gum and soil, were screened for their ability to transform beta-pinene to alpha-pinene. Biotransformation products were extracted with n-hexan and analyzed by gas chromatography. One microorganism (bacterial strain) were found. The biotransformation medium involved, phosphate buffer pH 6, 100 microL beta-pinene, 1 g biomass of microorganism, 37 degrees C, 150 rpm and 22 h. The experiments were performed in conical flasks. The optimum cell growth were obtained when 30 g L(-1) glycerin applied. The optimum conversion beta-pinene to alpha-pinene was obtained when 20 g L(-1) glycerin applied as carbon source for bacterial strain.
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| http://dx.doi.org/10.3923/pjbs.2007.1685.1690 | DOI Listing |
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