AI Article Synopsis
- Microinjection is a key method for introducing DNA into Caenorhabditis elegans, facilitating genetic transformation.
- The chapter outlines the reproductive biology of C. elegans and how it relates to microinjection techniques.
- It provides detailed applications and protocols for successful germline transformation to create transgenic worms.
Article Abstract
Microinjection is a commonly used technique for DNA transformation in Caenorhabditis elegans. It is a powerful tool that links genetic and molecular analysis to phenotypic analysis. In this chapter we shall provide an overview of microinjection for germline transformation in worms. Our discussion will emphasize C. elegans reproductive biology, applications and protocols for carrying out microinjection in order to successfully obtain transgenic worms.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796118 | PMC |
| http://dx.doi.org/10.1007/978-1-59745-202-1_10 | DOI Listing |
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