A new approach for label-free optical sample detection via surface enhanced Raman spectroscopy (SERS) inside a free-flow electrophoresis (FFE) chip is presented in this work.
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Microfluidic free-flow zone electrophoresis and isotachophoresis using carbon black nano-composite PDMS sidewall membranes.
Electrophoresis
January 2017
Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
We present a new type of free-flow electrophoresis (FFE) device for performing on-chip microfluidic isotachophoresis and zone electrophoresis. FFE is performed using metal gallium electrodes, which are isolated from a main microfluidic flow channel using thin micron-scale polydimethylsiloxane/carbon black (PDMS/CB) composite membranes integrated directly into the sidewalls of the microfluidic channel. The thin membrane allows for field penetration and effective electrophoresis, but serves to prevent bubble generation at the electrodes from electrolysis.
View Article and Find Full Text PDFIsotachophoretic free-flow electrophoretic focusing and SERS detection of myoglobin inside a miniaturized device.
Analyst
January 2009
Institute for Analytical Sciences, Bunsen-Kirchhoff-Strasse 11, 44139, Dortmund, Germany.
A new approach for label-free optical sample detection via surface enhanced Raman spectroscopy (SERS) inside a free-flow electrophoresis (FFE) chip is presented in this work.
View Article and Find Full Text PDFIsotachophoresis in free-flow using a miniaturized device.
Anal Chem
June 2006
Institute for Analytical Sciences Dortmund and Berlin, Bunsen-Kirchhoff-Strasse 11, D-44139 Dortmund, Germany.
For the first time, we report a miniaturized approach for isotachophoresis employing the technique of free-flow electrophoresis. Using a micromachined separation chamber with a volume of 200 nL, a sample mixture of fluorescein, eosin G, and acetylsalicylic acid was separated, stacked, and concentrated in less than a minute. Additionally, an isotachophoretic separation of a reaction mixture of myoglobin and fluoresceinisothiocyanate as a fluorescence label has shown the potential of this method for on-line sample preparation.
View Article and Find Full Text PDFSeparation and purification of methadone enantiomers by continuous- and interval-flow electrophoresis.
Anal Chem
May 1999
Institut für Anorganische und Analytische Chemie und Radiochemie, Fachrichtung 11.4 der Universität des Saarlandes, Postfach 151150, D-66041 Saarbrücken, Germany, Dr. Weber GmbH, Klausnerring 17, D-85551 Kirchheim, Germany, and Department of Clinical Pharmacology, University of Bern, Murtenstrasse 35, CH-3010 Bern, Switzerland.
Continuous- or free-flow electrophoresis is based upon a thin film of fluid flowing between two parallel plates. The electrolytes and the sample are continuously admitted at one end of the electrophoresis chamber and are fractionated by an array of outlet tubes at the other. Using the Octopus apparatus in a horizontal position, continuous preparative separation of methadone enantiomers in the presence of (2-hydroxypropyl)-β-cyclodextrin as a chiral selector was investigated under conditions of continuous-flow zone electrophoresis and continuous-flow isotachophoresis.
View Article and Find Full Text PDFAnalytical capillary isotachophoresis of human serum lipoproteins.
Electrophoresis
September 1997
Institut für Klinische Chemie und Laboratoriumsmedizin, Universität Regensburg, Germany.
An analytical free flow capillary isotachophoresis (cITP) procedure for the detailed analysis of lipoproteins on commercially available capillary electrophoresis systems has been developed. The technique is based on the specific staining of lipoproteins with the fluorescent lipophilic dye 7-nitrobenz-2-oxa-1,3-diazole (NBD)-ceramide before separation. Prestained lipoprotein samples are applied between leading and terminating buffer and separated into 9 well-characterized subpopulations according to their electrophoretic mobility in the absence of any molecular sieve effect.
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