In the cyanobacterial RuBisCO operon from Thermosynechococcus elongatus the rbcX gene is juxtaposed and cotranscribed with the rbcL and rbcS genes which encode large and small RuBisCO subunits, respectively. It has been suggested that the rbcX position is not random and that the RbcX protein could be a chaperone for RuBisCO. In this study, the RbcX protein from T. elongatus was overexpressed, purified and preliminary functional studies were conducted. The recombinant protein purified from Escherichia coli extracts was predominantly present in a soluble fraction in a dimeric form. Coexpression experiments have demonstrated that RbcX can mediate RbcL dimer (L(2)) formation, and that it is essential for the L(8) core complex assembly. This is the first characterization of the RbcX protein from a thermophilic organism.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Unique biogenesis and kinetics of hornwort Rubiscos revealed by synthetic biology systems.
Mol Plant
December 2024
School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, USA; Department of Cell and Molecular Biology, Uppsala University, 751 24 Uppsala, Sweden. Electronic address:
Hornworts are the only land plants that employ a pyrenoid to optimize Rubisco's CO fixation, yet hornwort Rubisco remains poorly characterized. Here we assembled the hornwort Anthoceros agrestis Rubisco (AaRubisco) using the Arabidopsis thaliana SynBio expression system and observed the formation of stalled intermediates, prompting us to develop a new SynBio system with A. agrestis cognate chaperones.
View Article and Find Full Text PDFThe first reported use of red blood cell exchange to treat hemoglobin Evans with secondary methemoglobinemia.
J Clin Apher
December 2023
Department of Hematology/Oncology, Mayo Clinic Hospital, Phoenix, Arizona, USA.
This manuscript describes a novel approach for treating patients with long-term sequelae from hemoglobin Evans (Hb Evans). After instituting conservative therapies for approximately 2 years, our patient's symptoms continually worsened. Therefore, we performed red blood cell exchange (RBCx) to reduce his Hb Evans percentage and his co-existing elevation of methemoglobin.
View Article and Find Full Text PDFHemoglobin S target of <50% as compared to 30% in chronic red cell exchange for secondary stroke prevention in sickle cell disease.
J Clin Apher
December 2023
Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
Article Synopsis
- Sickle cell disease patients with a history of stroke are generally advised to maintain a hemoglobin S (HbS) level below 30% to prevent strokes, but a new chronic exchange protocol by UT Southwestern allows for a higher target of below 50% for low-risk patients.
- A retrospective study reviewed medical records of 49 patients over 10 years, finding that 33 maintained an average HbS level of 35.4% without any strokes or transient ischemic attacks linked to this higher target.
- The results suggest that relaxing the HbS target may provide a flexible treatment option for certain patients without increasing stroke risk, although further research is needed to confirm these findings.
Red blood cell exchange in children with sickle cell disease.
Int J Hematol
January 2022
Centre Hospitalier de Cayenne, Rue des Flamboyants, BP 6006, 97306, Cayenne Cedex, French Guiana.
The aim of our study was to assess the efficacy of red blood cell exchange (RBCx) using a Spectra Optia® automated apheresis system in children with sickle cell disease (SCD). We used automated RBCx to treat acute and chronic complications in 75 children with SCD who had a median age of 10 years [7-13]. We analyzed 649 RBCx sessions.
View Article and Find Full Text PDFRuBisCO (Ribulose 1,5 bisphosphate carboxylase/oxygenase) by virtue of its dual specificity towards oxygen and carbon dioxide is an important rate-limiting step in photosynthesis and is believed to be the key factor for limited productivity of higher plants and algae. The photoautotrophic growth rate of cyanobacteria is a culmination of several factors including, rates of photosynthetic reactions, stress combating mechanisms and basic biomass generation metabolism in combination with optimal nutrient availability, irradiance, gaseous environment, etc. In case of cyanobacteria, the effect of RuBisCO in affecting the multiplication rate has been observed to show varied response.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!