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Analysis of angiographic findings and short-term recurrence factors in patients presenting with hemoptysis.
Biomed Eng Online
August 2024
Department of Interventional Medicine, First Hospital of Lanzhou University, Lanzhou City, Gansu Province, China.
Objectives: The abnormal anatomical alterations of blood vessels during DSA angiography in patients with hematological disorders were retrospectively examined, and the influencing factors of short-term (≤ 6 months) recurrent hemoptysis were statistically analyzed, and the consistency between admission diagnosis and intraoperative diagnosis was evaluated.
Methods: The intraoperative angiography data of patients who underwent selective bronchial artery embolization for hemoptysis in our hospital from January 2022 to December 2022 were reviewed. They were divided into the observation group and the control group based on whether there was recurrent hemoptysis.
Development and evaluation of a triplex droplet digital PCR method for differentiation of , and BCG.
Front Microbiol
June 2024
National Animal Tuberculosis Reference Laboratory, Division of Zoonoses Surveillance, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Article Synopsis
- Tuberculosis caused by Mycobacterium tuberculosis complex (MTBC) remains a major global health issue, emphasizing the need for effective detection methods to differentiate its main pathogens.
- The study introduces a new testing method called triplex droplet digital polymerase chain reaction (ddPCR), which uses different probe fluorophores to accurately target genes associated with three pathogens: MTBC, its variants, and BCG.
- The results show that triplex ddPCR is more sensitive and specific than current methods, detecting lower levels of pathogens in various sample types (including milk, tissue, and swabs) with high repeatability and without cross-reaction to other organisms.
The diagnostic value of bronchoalveolar lavage fluid metagenomic next-generation sequencing in critically ill patients with respiratory tract infections.
Microbiol Spectr
August 2024
Medical Laboratory of Jining Medical University, Lin He's Academician Workstation of New Medicine and Clinical Translation in Jining Medical University, Jining Medical University, Shandong Jining, China.
Article Synopsis
- Metagenomic next-generation sequencing (mNGS) is a quick and unbiased method for identifying pathogens in patients with severe pneumonia, showing superior sensitivity (97.54%) compared to conventional microbiological tests (28.68%).
- The study involved 145 patients and revealed that while mNGS had higher sensitivity and negative predictive value, it had lower specificity than conventional methods (52.17% vs 87.5%).
- mNGS effectively identified a wide range of pathogens, including atypical ones, and led to significant clinical improvements in most patients (95.86%) after adjusting antibiotics based on its results.
Exploring the accuracy of third-generation Nanopore Sequencing technology for detecting mycobacterium tuberculosis in patients with diabetes mellitus.
Diagn Microbiol Infect Dis
September 2024
Department of Tuberculosis, The Fourth People's Hospital of Nanning, NO.1 Changgang-two-li Road, Nanning, Guangxi, China, 530023. Electronic address:
Article Synopsis
- The study aimed to assess how effective third-generation nanopore sequencing is for diagnosing pulmonary tuberculosis (TB) in patients with diabetes mellitus suspected of having the disease.
- Researchers collected samples from patients and compared nanopore sequencing results to traditional diagnostic methods like acid-fast bacilli smear and Xpert MTB/RIF.
- Findings showed that nanopore sequencing significantly enhanced diagnostic accuracy and sensitivity, especially when used in combination with Xpert testing, indicating it could be a better option for TB detection in diabetic patients.
Individual- and Herd-Level Milk ELISA Test Status and Incidence for Paratuberculosis in Hubei Province, China.
Vet Sci
May 2024
National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
subsp. (MAP) is responsible for the persistent infectious illness known as bovine paratuberculosis, which is one of the most easily overlooked diseases in China amid a lack of epidemiological data. In this study, we evaluated the agreement of milk and blood antibody tests for paratuberculosis and showed an overall agreement of 92.
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