We previously reported assembly and cloning of the synthetic Mycoplasma genitalium JCVI-1.0 genome in the yeast Saccharomyces cerevisiae by recombination of six overlapping DNA fragments to produce a 592-kb circle. Here we extend this approach by demonstrating assembly of the synthetic genome from 25 overlapping fragments in a single step. The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2600582 | PMC |
| http://dx.doi.org/10.1073/pnas.0811011106 | DOI Listing |
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