AI Article Synopsis
- The study analyzed the MSTN gene's UTR polymorphism in 345 sheep from 11 different breeds using PCR-RFLP methods.
- Two PCR products (271 bp and 1,003 bp) were digested, revealing significant polymorphism among the breeds, with a notable variation in genotype distribution.
- Findings showed that 9 local sheep breeds differed from Texel sheep at a specific SNP site linked to muscularity, and sequencing revealed a high mutation frequency in the 3'UTR region that could affect miRNA motifs.
Article Abstract
PCR-RFLP was applied to analyze the polymorphism of MSTN gene UTR in 345 sheep that comprised of eleven sheep breeds, namely Texel sheep, Charolais sheep, Small-tailed Han sheep, Monggolian sheep, Ujumqin sheep, Altay Fat-rumped sheep, Hulunbeir sheep, Tashikurgan sheep, Duolang sheep, Hu sheep, and Gangba sheep. A 271 bp and a 1 003 bp long PCR products were digested with Mboand Bsato demonstrate polymorphism in the eleven sheep breeds, which were all at Hardy-Weinberg equilibrium (P>0.05). The distribution of 3 genotypes in 11 sheep breeds was significantly different (P<0.01). Digestion of the PCR products with HpyCH4 proved that 9 domestic local sheep breeds were different from Texel sheep in the SNP site that was associated with muscularity. The individual mutation base could generate the motifs for miRNA in the 3'UTR, and sequencing analysis demonstrated high frequency of mutation in the 3'UTR region.
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| http://dx.doi.org/10.3724/sp.j.1005.2008.01585 | DOI Listing |
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