Chemiluminescence detector with a serpentine flow cell.

Anal Chem

School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3217, Australia.

Published: December 2008

We present a new chemiluminescence detector, with solution channels that have been machined into a Teflon disk and sealed with a sapphire window. The configuration of the flow cell can be conveniently modified by replacing the Teflon disk. A comparison of some existing and novel designs, using the chemiluminescence reaction of morphine with acidic potassium permanganate and the bioluminescence reaction of ATP with the commercially available "BacTiter-Glo" reagent, has revealed that a serpentine channel allows greater quantities of light to be captured than a spiral channel, due to more efficient mixing of the analyte and reagent solutions within the cell.

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Source
http://dx.doi.org/10.1021/ac801842qDOI Listing

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