We have previously shown that P7 rat pups injected with the N-methyl-d-aspartate receptor (NMDAR) blocker MK801 displayed robust apoptotic injury within hours after injection. Further studies from our lab suggest that loss of calcium cannot be compensated for when vulnerable neurons lack calcium buffering capabilities. Thus, to elevate calcium in these neurons prior to MK801 exposure, we injected P7 rats with the calcium channel agonist BayK 8644. Whereas BayK 8644 did not induce apoptosis by itself, it was found to block MK801-induced injury in a dose-dependent manner. Reversal of MK801 toxicity was complete in the caudate-putamen, partial in the somatosensory cortex but was not observed in the retrosplenial cortex. These results suggest that postnatal brain injury resulting from agents that block the NMDAR, which include commonly used anesthetics as well as drugs of abuse, may be prevented in vulnerable neurons by compensatory increases in calcium prior to exposure to these antagonists.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2699449 | PMC |
| http://dx.doi.org/10.1016/j.neulet.2008.12.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Biphasic effect of limonene on contraction of isolated rat aorta.
Chem Biol Interact
January 2025
Department of Biological Chemistry, Regional University of Cariri, 63105-000, Crato, CE, Brazil.
Article Synopsis
- Limonene, a compound in essential oils, demonstrates various health benefits like antifungal and antioxidant properties, but its effects can vary based on concentration, showing a biphasic response.
- The study investigates limonene's impact on rat aorta contractions, revealing it can enhance or inhibit contractions depending on the dose, particularly with phenylephrine.
- Understanding these dose-dependent effects is crucial for determining safe and effective therapeutic uses of limonene in clinical settings.
Vasomotion in human arteries and their regulations based on ion channel regulations: 10 years study.
J Cell Physiol
September 2023
Department of Surgery, College of Medicine, Chungbuk National University Hospital (CBNUH), Chungbuk National University (CBNU), Cheongju, Chungbuk, Korea.
Vasomotion is the oscillation of vascular tone which gives rise to flow motion of blood into an organ. As is well known, spontaneous contractile organs such as heart, GI, and genitourinary tract produce rhythmic contraction. It imposes or removes pressure on their vessels alternatively for exchange of many substances.
View Article and Find Full Text PDFSensitivity of the S1 neuronal calcium network to insulin and Bay-K 8644 in vivo: Relationship to gait, motivation, and aging processes.
Aging Cell
July 2022
Department of Pharmacology and Nutritional Sciences, University of Kentucky, Lexington, Kentucky, USA.
Neuronal hippocampal Ca dysregulation is a critical component of cognitive decline in brain aging and Alzheimer's disease and is suggested to impact communication and excitability through the activation of a larger after hyperpolarization. However, few studies have tested for the presence of Ca dysregulation in vivo, how it manifests, and whether it impacts network function across hundreds of neurons. Here, we tested for neuronal Ca network dysregulation in vivo in the primary somatosensory cortex (S1) of anesthetized young and aged male Fisher 344 rats using single-cell resolution techniques.
View Article and Find Full Text PDFThe role of bicarbonate in the modulation of capacitation, spontaneous acrosome reaction and motility of equine fresh and frozen spermatozoa.
Theriogenology
July 2022
Department of Emergency and Organs Transplantation, University of Bari Aldo Moro, Italy.
In this study, we defined the composition of the culture medium that yield a significant percentage of alive and functional equine spermatozoa during enough time before artificial insemination. The effects of sodium bicarbonate were analyzed in fresh and frozen semen in respect to sperm viability, capacitation, spontaneous acrosome reaction and several kinetic parameters such as total motility, progressive motility, VCL, VSL, ALH, BCF, LIN. Moreover, employing Bayk-6844 and Nifedipine, the involvement of L-type voltage-gated calcium channels in the modulation of intracellular calcium concentrations was investigated.
View Article and Find Full Text PDFSimultaneous imaging of calcium and contraction in the beating heart of zebrafish larvae.
Theranostics
April 2022
Physiology and Cell Dynamics, Centro Regional de Investigaciones Biomédicas (CRIB) and Facultad de Medicina de Albacete, Universidad de Castilla-La Mancha, C/Almansa 14, 02006 Albacete, Spain.
models of cardiac function maintain the complex relationship of cardiomyocytes with other heart cells, as well as the paracrine and mechanoelectrical feedback mechanisms. We aimed at imaging calcium transients simultaneously with heart contraction in zebrafish larvae. To image calcium in beating hearts, we generated a zebrafish transgenic line expressing the FRET-based ratiometric biosensor Twitch-4.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!