AI Article Synopsis
- The study investigates how recombinant thymosin beta4 (Tbeta4) promotes skin wound healing in rats by affecting laminin 5 expression.
- A total of 60 rats were divided into control and experimental groups, with Tbeta4 applied in varying doses post-wound creation, and healing was monitored over seven days.
- Results showed improved wound healing rates in Tbeta4-treated groups compared to the control, with significant differences noted after 2 days, and increased expression of laminin 5 linked to cellular proliferation and differentiation.
Article Abstract
Objective: To explore the mechanism of recombinant thymosin beta4 (Tbeta4) accelerating skin wound healing in rats by regulating laminin 5 expression.
Methods: Two full thickness 8 mm punch wounds were made at the costovertebral angle on dorsal surface of each adult male rats weighing 200-250 g. Sixty rats were randomized into the control group (n = 15) and the experimental group (n = 45), which was subdivided into low, medium and high dose groups (n = 15). Tbeta4 was applied topically at 2, 6, 18 microg in 50 microL PBS for every 12 hours after model making in the experimental group. The identical amounts of phosphate buffered saline was applied in the control group. Wound healing was observed after model making and immunohistochemical observation was conducted 2, 4 and 7 days after operation.
Results: Seven days after operation, wound contracted obviously and most of the wounds connected well with the margin. In the control group, low dose group, medium dose group and high dose group, the wound healing rate were 7.67% +/- 5.46%, 29.01% +/- 7.43%, 26.54% +/- 11.49% and 10.39% +/- 3.96% respectively 2 days after operation; 28.16% +/- 13.76%, 37.99% +/- 13.05%, 42.00% +/- 9.56% and 39.58% +/- 12.74% respectively 4 days after operation; 59.08% +/- 19.02%, 64.15% +/- 17.92%, 77.39% +/- 8.45% and 69.78% +/- 8.45% respectively 7 days after operation. At 2 days after operation, significant differences were notified in healing rats between 3 sub-experimental groups and the control group (P < 0.05). Immunohistochemistry staining showed that there was a little more positive expression of laminin 5 2 days after operation that beneficial to promote the proliferation and differentiation of cell in every group, including positive cells and ECM. But in medium group there was fewer expression, only at the borderline and bottom of the wound, while the expression significantly increased 4 days after operation (P < 0.05) and there was a relative high expression 7 days after operation (P < 0.01).
Conclusion: Tbeta4 can inhibit the expression of laminin 5 early, and then up-regulate laminin 5 expression to moderate the reformation of ECM, promote the migration of epidemic cell and accelerate skin wound healing.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Effectiveness of Synchronous Postdischarge Contacts on Health Care Use and Patient Satisfaction : A Systematic Review and Meta-analysis.
Ann Intern Med
January 2025
Center of Innovation to Accelerate Discovery and Practice Transformation, Durham Veterans Affairs Health Care System; Department of Population Health Sciences, Duke University School of Medicine; and Durham Evidence Synthesis Program, Durham Veterans Affairs Health Care System, Durham, North Carolina (J.M.G.).
Background: Postdischarge contacts (PDCs) after hospitalization are common practice, but their effectiveness in reducing use of acute care after discharge remains unclear.
Purpose: To assess the effects of PDC on 30-day emergency department (ED) visits, 30-day hospital readmissions, and patient satisfaction.
Data Sources: MEDLINE, Embase, and CINAHL searched from 2012 to 25 May 2023.
Effects of Recording Condition and Number of Monitored Days on the Discriminative Power of the Daily Phonotrauma Index.
J Speech Lang Hear Res
January 2025
Center for Laryngeal Surgery and Voice Rehabilitation, Massachusetts General Hospital, Boston.
Purpose: The Daily Phonotrauma Index (DPI) can quantify pathophysiological mechanisms associated with daily voice use in individuals with phonotraumatic vocal hyperfunction (PVH). Since DPI was developed based on weeklong ambulatory voice monitoring, this study investigated if DPI can achieve comparable performance using (a) short laboratory speech tasks and (b) fewer than 7 days of ambulatory data.
Method: An ambulatory voice monitoring system recorded the vocal function/behavior of 134 females with PVH and vocally healthy matched controls in two different conditions.
Discharge Disposition after Total Hip Arthroplasty: A 10-Year Analysis of Trends and Predictors of Nonhome Discharge (2011-2021).
J Am Acad Orthop Surg
January 2025
From the Department of Orthopedic Surgery, Cleveland Clinic Foundation, Cleveland, OH (Pasqualini, Ibaseta, T Khan, and Piuzzi), the Case Western Reserve University School of Medicine, Cleveland, OH (Pan, Xu, and Austin), the Department of Orthopaedic Surgery, Larkin Community Hospital, South Miami, FL (Corces), and Levitetz Department of Orthopaedic Surgery, the Cleveland Clinic Florida, Weston, FL (Higuera).
Background: Total hip arthroplasty (THA) practices are evolving under the influence of the current value-based healthcare system and bundled payment models. This study aimed to (1) evaluate national trends in discharge disposition and postoperative outcomes after THA, (2) compare discharge cohorts on episode-of-care parameters, and (3) determine predictors of nonhome discharge from 2011 to 2021.
Methods: The National Surgical Quality Improvement Program database was queried for THA data from 2011 to 2021.
Early therapy contributes to the normalization of platelet in patients with severe fever with thrombocytopenia syndrome during the convalescent phase.
PLoS Negl Trop Dis
January 2025
Department of Infectious Disease, Peking University Ditan Teaching Hospital, Beijing, China.
Background: Platelet recovery was an important prognostic indicator in severe fever with thrombocytopenia syndrome (SFTS). This study focused on risk factors affecting platelet recovery in surviving SFTS patients, which can assist clinicians in the early screening of patients associated with a greater risk of mortality.
Method: We retrospectively analyzed the clinical data of SFTS patients admitted to Yantai Qishan Hospital throughout 2023.
Cardiorespiratory demands of firearms training instruction and 15m shuttle tests in British law enforcement.
PLoS One
January 2025
Division of Surgery & Interventional Science, Institute of Sport Exercise and Health, University College London, London, United Kingdom.
Objectives: Law enforcement agencies require minimum fitness standards to safeguard their officers and training staff. Firearms instructors (FI) are expected to maintain the same standards as their operational counterparts. This study aimed to quantify the daily physiological demands placed on FI.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!