The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. Described here is a drop plaque assay, which, being simpler, faster and more efficient than either the classical overlay or direct plating methods, enhances efficiency in processing large numbers of samples.
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http://dx.doi.org/10.1007/978-1-60327-164-6_9 | DOI Listing |
SAGE Open Med Case Rep
October 2024
Division of Dermatology, University of Calgary, Calgary, AB, Canada.
Nail psoriasis occurs in approximately half of all cases of plaque psoriasis and manifests with onychodystrophy, which includes morphological features of onycholysis, subungual hyperkeratosis, oil drop sign, pitting, splinter hemorrhages, leukonychia, and crumbling of the nails. Nail psoriasis can have a significant adverse impact on quality of life. However, nail psoriasis is often refractory to both local and systemic therapies, making it challenging to treat.
View Article and Find Full Text PDFJ Conserv Dent Endod
August 2024
Department of Conservative Denstistry and Endodontics, Desh Bhagat Dental College and Hospital, Mandigobindgarh, Punjab, India.
Aim: The aim of this study is to assess the effect of selected locally available beverages on salivary pH, flow rate, and oral clearance rate among adults.
Materials And Methods: A quantitative diagnostic method for dental caries would improve oral health, which directly affects the quality of life. The pH scale measures how acidic or basic a solution is, with 7 being neutral.
Biofilm
December 2024
Institute of Dentistry, University of Turku, Lemminkaisenkatu 2, 20520, Turku, Finland.
Background: Acidogenicity and production of an extracellular matrix (ECM) are important virulence factors for the dental caries-associated bacteria, such as that live in biofilms on tooth surface. The ECM protects the bacteria from the flushing and buffering effects of saliva resulting in highly acidic microenvironments inside the biofilm.
Materials And Methods: In this study, we applied real-time assays to follow biofilm formation and pH decrease in a growth medium and saliva by three strains, as well as acid neutralization inside the mature biofilm.
Clin Cosmet Investig Dent
September 2024
ANCOSTAT Statistical Consulting, San Antonio, Texas, USA.
Purpose: This study investigated the influence of nanohydroxyapatite-containing (nanoHAP) lozenge on plaque pH following sucrose intake.
Patients And Methods: Sixteen adult subjects were enrolled in this double-blind crossover study composed of four interventions: (1) 10% w/v sucrose solution, (2) 10% w/v sorbitol solution, (3) nanoHAP lozenge, and (4) 10% w/v sucrose solution challenge followed by nanoHAP lozenge. Following the determination of each subject's resting plaque pH, the pH was measured at different time intervals from 3 to 30 minutes from the start of intervention, with 7 days interval between the applications of different interventions.
Biomed Phys Eng Express
August 2024
Department of Mechanical Engineering, Tezpur University, Assam, 784028, India.
Vascular diseases are greatly influenced by the hemodynamic parameters and the accuracy of determining these parameters depends on the use of correct boundary conditions. The present work carries out a two-way fluid-structure interaction (FSI) simulation to investigate the effects of outlet pressure boundary conditions on the hemodynamics through the left coronary artery bifurcation with moderate stenosis (50%) in the left anterior descending (LAD) branch. The Carreau viscosity model is employed to characterise the shear-thinning behaviour of blood.
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