Enumeration of bacteriophages by the direct plating plaque assay.

Methods Mol Biol

Public Health Agency of Canada, Laboratory for Foodborne Diseases, Guelph, Ontario, Canada.

Published: March 2009

A method is described for determination of the concentration of infectious phage particles by the direct plating plaque assay, which is simpler and faster than the double agar overlay plaque procedure outlined in the previous chapter.

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-60327-164-6_8DOI Listing

Publication Analysis

Top Keywords

direct plating
8
plating plaque
8
plaque assay
8
enumeration bacteriophages
4
bacteriophages direct
4
assay method
4
method described
4
described determination
4
determination concentration
4
concentration infectious
4

Similar Publications

Serum metabolic fingerprinting on Ag@AuNWs for traumatic brain injury diagnosis.

Nanotechnology

January 2025

Xi'an Jiaotong University, xian ning west road 28#, xi'an, Xi'an, None Selected, 710049, CHINA.

Accurate and rapid diagnosis of traumatic brain injury (TBI) is essential for high-quality medical services. Nonetheless, the current diagnostic platform still has challenges in rapidly and accurately analysing clinical samples. Here, we prepared a highly stable, repeatable and sensitive gold-plated silver core-shell nanowire (Ag@AuNWs) for surface-enhanced Raman spectroscopy (SERS) metabolic fingerprint diagnosis of TBI.

View Article and Find Full Text PDF

Introduction BioGlue® (CryoLife, Inc, Kennesaw, GA), despite being claimed to be a safe and harmless sealant, reportedly has several adverse effects including surgical wound dehiscence. This study aimed to examine the factors that may contribute to this unfavorable outcome in cranial surgery. Methods A retrospective cross-sectional analysis was conducted on patients who underwent brain surgery with the use of BioGlue® between January 2015 and December 2022.

View Article and Find Full Text PDF

Background: Improvements in cementless total hip arthroplasty have been directed at optimizing osseointegration of the femoral implant to reduce aseptic loosening rates. Stem design plays a critical role in the performance of these implants. Given the increase in new stem designs and the creation of an updated classification system, improved understanding of the outcomes of each stem type is warranted.

View Article and Find Full Text PDF

Background: To explore the advantages of a lateral tibial locking plate combined with Jail screw fixation in the treatment of anterolateral tibial plateau collapse fracture (ATPCF).

Methods: A retrospective analysis was conducted on patients with ATPCFs admitted to our hospital from February 2019 to February 2023. Twenty-six patients were successfully included, including 15 males and 11 females, with an average age of 58.

View Article and Find Full Text PDF

The directivity of the quasi-static component (QSC) is quantitatively investigated for evaluating the orientation of a micro-crack buried in a thin solid plate using the numerical simulation method. Based on the bilinear stress-strain constitutive model, a three-dimensional (3D) finite element model (FEM) is built for investigating the nonlinear interaction between primary Lamb waves and the micro-crack. When the primary Lamb waves at A0 mode impinge on the micro-crack, under the modulation of the contact acoustic nonlinearity (CAN), the micro-crack itself will induce QSC.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!