AI Article Synopsis
- This study examines how various experimental factors impact polarization responses in two-photon inverted microscopy, focusing on the effects of reflection optics and high numerical aperture focusing.
- The researchers found that these factors can distort polarization responses, potentially leading to inaccurate readings of molecular order from polarimetric measurements.
- A new model is proposed to account for these distortions, along with a calibration technique for accurately determining polarization parameters in sample liquids during two-photon fluorescence polarimetry.
Article Abstract
We present a global analysis of experimental factors affecting polarization responses in two-photon inverted microscopy. The role of reflection optics and high numerical aperture focusing is investigated in two-photon fluorescence, which can be extended to other nonlinear processes. We show that both effects strongly distort polarization responses and can lead to misleading extraction of molecular order information from polarimetric measurements. We describe a model accounting for these effects and develop a calibration technique for the determination of polarization parameters in the sample plane using two-photon fluorescence polarimetry in liquids.
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| http://dx.doi.org/10.1364/oe.16.020891 | DOI Listing |
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