We have developed a novel referencing technique for surface plasmon resonance imaging systems referred to as "spot and hop." The technique enables internal referencing for individual flow cells in a parallel processing microfluidic network. Internal referencing provides the ability to correct for nonspecific binding and instrument drift, significantly improving data quality at each region of interest. The performance of a 48-flow-cell device was demonstrated through a series of studies, including "rise and fall" time, ligand preconcentration, ligand immobilization, analyte binding, and regeneration tests. Interfacing parallel processing fluidics with imaging systems will significantly expand the throughput and applications of array-based optical biosensors while retaining high data quality.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691735 | PMC |
| http://dx.doi.org/10.1016/j.ab.2008.11.014 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Assessing Active and Passive Glenohumeral Rotational Deficits in Professional Tennis Players: Use of Normative Values at 90° and 45° of Abduction to Make Decisions in Injury-Prevention Programs.
Sports (Basel)
December 2024
Facultat de Psicologia Ciències de l'Educació i l'Esport (FPCEE) Blanquerna, Universitat Ramon Llull, 08022 Barcelona, Spain.
(1) Background: Glenohumeral internal and external rotational range-of-movement deficits (GIRDs and GERDs) are some of the primary outcomes used to determine the risk of injury in overhead athletes, such as tennis players. Nevertheless, the current testing position does not consider the fact that most tennis actions are repeated at 45° of abduction, and actively. The aim of this study was to establish normative values of pathological GIRDs and GERDs in tennis players and to provide normative values for both the passive and active rotational range of motion of the glenohumeral joint at 90° and 45° of abduction.
View Article and Find Full Text PDFExploratory Studies on RNAi-Based Therapies Targeting Angiotensinogen in Hypertension: Scoping Review.
J Pers Med
December 2024
Medicine School, Pontifical Catholic University of Goiás, Goiânia 74605-010, Brazil.
Systemic arterial hypertension contributes to cardiovascular morbidity and mortality worldwide. Many patients cannot achieve optimal blood pressure (BP) control with traditional therapies, which often results in poor patient adherence and limited long-term efficacy. We investigated the potential of RNA interference (RNAi) therapies targeting hepatic angiotensinogen (AGT) for hypertension management.
View Article and Find Full Text PDFA novel overtone peak self-referencing fluorescent sensor based on a bipyridine-linked covalent organic framework for highly sensitive copper ion detection.
Anal Methods
January 2025
Jiangxi University of Chinese Medicine, Nan Chang, Jiangxi 330004, China.
This study reports a novel ratiometric fluorescence sensor based on a tetraphenylethylene-bipyridine covalent organic framework (TPE-Bpy-COF) for the sensitive detection of Cu, leveraging the unique coordination properties of the bipyridine moieties. The interaction between Cu and the nitrogen atoms in the bipyridine units induces fluorescence quenching at 500 nm through an efficient host-guest electron transfer mechanism, where excited-state electrons from the COF framework are transferred to the vacant orbitals of Cu. Upon excitation at 410 nm, the sensor exhibits a primary emission peak at 500 nm, which is quenched in the presence of Cu, while an overtone peak at 820 nm remains stable, serving as an internal reference for ratiometric measurements and significantly enhancing the accuracy and reliability of the sensor.
View Article and Find Full Text PDFMortality and reoperations following treatment of acetabular fractures in patients ≥ 70 years: a retrospective cohort study of 247 patients.
Acta Orthop
January 2025
Department of Surgical Sciences, Section for Orthopaedics, Uppsala University, Uppsala, Sweden.
Background And Purpose: Evidence for long-term outcomes following acetabular fractures in older adults is limited. We aimed to evaluate mortality, complications, and need for subsequent surgical procedures in operatively and nonoperatively treated older patients with acetabular fractures.
Methods: Patients aged ≥ 70 years with acetabular fractures treated at Uppsala University Hospital between 2010 and 2020 were included.
Evaluating case definitions of respiratory disease in dairy calves: a scoping review.
J Dairy Sci
January 2025
Department of Population Medicine, University of Guelph, Guelph, ON, Canada N1G 2W1. Electronic address:
Bovine respiratory disease (BRD) is one of the most common diseases observed in dairy calves in both the pre- and post-weaning periods. Despite its common occurrence, there has been no formal synthesis (e.g.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!