Here, we identify a cluster of eight genes on chromosome 2 of Agrobacterium vitis that is associated with the ability of the bacterium to cause a hypersensitive response on tobacco and a necrosis of grape shoot explants. Three of these genes share a high level of structural and sequence similarity to clusters of genes in other bacteria that encode the enzymes for biosynthesis of polyketides and long-chain polyunsaturated fatty acids. No similar gene clusters were discovered in sequenced genomes of other members of Rhizobiales.
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http://dx.doi.org/10.1111/j.1574-6968.2008.01371.x | DOI Listing |
PeerJ
December 2024
The Thomas H. Gosnell School of Life Sciences, Biotechnology and Molecular Bioscience Program, College of Science, Rochester Institute of Technology, Rochester, New York, United States.
Background: A grapevine crown gall tumor strain, sp. strain Rr2-17 was previously reported to accumulate copious amounts of diverse quorum sensing signals during growth. Genome sequencing identified a single luxI homolog in strain Rr2-17, suggesting that it may encode for a AHL synthase with broad substrate range, pending functional validation.
View Article and Find Full Text PDFPlant Physiol Biochem
October 2024
Tianjin Agricultural University, College of Horticulture and Landscape Architecture, Tianjin, 300394, China. Electronic address:
Crown gall caused by Agrobacterium vitis (A. vitis) is one of the crucial issues restricting the to grape industry. In this study, Agrobacterium tumefaciens (Ag-8) was separated from the soil that could prevent the occurrence of grape crown gall.
View Article and Find Full Text PDFMicrobiol Resour Announc
August 2024
Program in Biotechnology and Molecular Bioscience, Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, New York, USA.
Using Nanopore Q20+ sequencing, we report the complete genome of () strain CG957=AA25, isolated nearly 40 years ago from a grapevine crown gall in Afghanistan. The assembled genome size is 6 Mb, comprising a circular chromosome, a linear chromid, a Ti plasmid, and two non-Ti plasmids.
View Article and Find Full Text PDFPlant Dis
December 2023
Agriculture and Agri-Food Canada, Summerland Research and Development Centre, Summerland, BC V0H 1Z0, Canada.
Crown gall disease of grapevines caused by causes significant damage to vineyards in cold-climate viticulture areas such as Canada and the northern United States. Introduction of the disease into vineyards occurs mainly through planting of infected but asymptomatic nursery material. Because is not a regulated pest for import into Canada, no information on the health status of nursery material destined for import into Canada has previously been collected.
View Article and Find Full Text PDFBMC Res Notes
July 2023
Carle Foundation Hospital, Urbana, IL, 61801, USA.
This work presents the design of a new protein based on the adenosine triphosphate-binding cassette (ABC) transporter solute binding protein (SBP) derived from Agrobacterium vitis, a gram-negative plant pathogen. The Protein Data Bank in Europe's dictionary of chemical components was utilized to identify sorbitol and D-allitol. Allitol bound to an ABC transporter SBP was identified in the Research Collaboratory for Structural Bioinformatics Protein Data Bank (RCSB).
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