[Measurement of airborne cladosporium spp antigens by aeroallergen immnoblotting and the seasonal fluctuation].

Background: Airborne Cladosporium spp (Clad) antigens were measured by aeroallergen immnoblotting, and analyzed the seasonal fluctuation of the antigens.

Methods: Airborne Clad antigens (Clad) were transferred onto nitrocellulose membrane and treated with anti-Clad rabbit antiserum, then alkaline phosphatase-conjugated rabbit IgG. Finally, the spots from Clad antigens were visualized with BCIP/NBT substrate.

Results: Clear purple spots from Clad antigens were seen on the airborne samples. Two peaks of spring and autumn were observed on the study of daily examination of the spots. Many spots from Clad antigens were mainly observed from afternoon to midnight at daily basis. No correlation between the number of the spots and the four weather elements was seen.

Conclusion: Spots from airborne Clad antigens can be obtained easily by use of an immunochemical technique. The great numbers of spores were seen in spring and autumn out of one year and from afternoon to midnight out of one day.