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Formin proteins of the DAAM subfamily play a role during axon growth. | LitMetric

AI Article Synopsis

  • The regulation of actin dynamics in growth cones is crucial for controlling axonal growth, and actin nucleation factors are key players in forming new actin filaments.
  • The study identifies the Drosophila formin DAAM as essential for axonal morphogenesis and filopodia formation, demonstrating its importance in developing neurons.
  • Genetic and protein interaction analyses reveal that DAAM works with Rac GTPases, Profilin, and Enabled, and its function appears evolutionarily conserved, as mouse Daam1 can compensate for defects in dDAAM mutant flies.

Article Abstract

The regulation of growth cone actin dynamics is a critical aspect of axonal growth control. Among the proteins that are directly involved in the regulation of actin dynamics, actin nucleation factors play a pivotal role by promoting the formation of novel actin filaments. However, the essential nucleation factors in developing neurons have so far not been clearly identified. Here, we show expression data, and use true loss-of-function analysis and targeted expression of activated constructs to demonstrate that the Drosophila formin DAAM plays a critical role in axonal morphogenesis. In agreement with this finding, we show that dDAAM is required for filopodia formation at axonal growth cones. Our genetic interaction, immunoprecipitation and protein localization studies argue that dDAAM acts in concert with Rac GTPases, Profilin and Enabled during axonal growth regulation. We also show that mouse Daam1 rescues the CNS defects observed in dDAAM mutant flies to a high degree, and vice versa, that Drosophila DAAM induces the formation of neurite-like protrusions when expressed in mouse P19 cells, strongly suggesting that the function of DAAM in developing neurons has been conserved during evolution.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6671601PMC
http://dx.doi.org/10.1523/JNEUROSCI.2727-08.2008DOI Listing

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