A DNA endonuclease has been purified from eggs of Asterias forbesi by a simple four-step-purification procedure. The purified enzyme is at least 96% pure and is free of phosphatase, phosphodiesterase, and RNase. It has a pH optimum of 6.5 and does not require divalent cations. The enzyme produces 3'-phosphoryl and 5'-hydroxyl end groups. The products of exhaustive hydrolysis can be grouped in two fractions. The first fraction, 40%, contains a small amount of mononucleotides and di-, tri-, tetra-, penta-, and hexanucleo-tides. The second fraction, 60%, contains oligonucleotides larger than hexanucleotides.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/bi00634a009 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Chemical Warfare in the Environment-Secondary Metabolites From Starfish (Asterias amurensis) Induce Teratogenicity in Medaka Embryos (Oryzias melastigma).
Chem Biodivers
November 2024
Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China.
Article Synopsis
- Starfish saponins have been studied for their ability to deter predators and their cytolytic effects, suggesting these compounds play significant roles in their ecological interactions.
- Recent research focused on secondary metabolites from the starfish Asterias amurensis, hypothesizing they may harm marine fish eggs, specifically medaka, by separating and identifying two harmful oligoglycosides.
- The identified compounds showed strong lethality and caused severe developmental issues in medaka embryos, highlighting potential ecological impacts on species sharing habitats with starfish.
Gonadotropic activity of a second relaxin-type peptide in starfish.
Gen Comp Endocrinol
November 2023
Department of Bioengineering, School of Engineering, Tokai University, 4-1-1, Kitakaname, Hiratsuka, Kanagawa, 259-1292, Japan.
In starfish, a relaxin-like gonad-stimulating peptide (RGP) acts as a gonadotropin that triggers gamete maturation and spawning. In common with other relaxin/insulin superfamily peptides, RGP consists of an A- and a B-chain, with cross-linkages mediated by one intra- and two inter-chain disulfide bonds. In this study, a second relaxin-like peptide (RLP2) was identified in starfish species belonging to the orders Valvatida, Paxillosida, and Forcipulatida.
View Article and Find Full Text PDFIn Silico Reconstruction of Sperm Chemotaxis.
Int J Mol Sci
August 2021
Department of Bioscience and Informatics, Keio University, Yokohama 223-8522, Japan.
In echinoderms, sperm swims in random circles and turns in response to a chemoattractant. The chemoattractant evokes transient Ca influx in the sperm flagellum and induces turning behavior. Recently, the molecular mechanisms and biophysical properties of this sperm response have been clarified.
View Article and Find Full Text PDFEffects of embryo energy, egg size, and larval food supply on the development of asteroid echinoderms.
Ecol Evol
July 2020
Biology Department William & Mary Williamsburg Virginia USA.
Organisms have limited resources available to invest in reproduction, causing a trade-off between the number and size of offspring. One consequence of this trade-off is the evolution of disparate egg sizes and, by extension, developmental modes. In particular, echinoid echinoderms (sea urchins and sand dollars) have been widely used to experimentally manipulate how changes in egg size affect development.
View Article and Find Full Text PDFReproductive characteristics and life-history relationships of starry smooth-hound Mustelus asterias in British waters.
J Fish Biol
December 2015
Centre for Environment, Fisheries and Aquaculture Science (CEFAS), Lowestoft Laboratory, Pakefield Road, Lowestoft, Suffolk NR33 0HT, U.K.
The reproductive biology and other life-history parameters were investigated for Mustelus asterias in British waters, with specimens caught from both commercial fisheries and research-vessel surveys. In total, 504 specimens [238 males, 24-99 cm total length (LT) and 266 females, 28-124 cm LT] were examined, with further information collected from 238 uterine pups. The lengths at 50% maturity were estimated as 70·4 and 81·9 cm LT for males and females, respectively.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!