The real-time atomic force microscope for biological sample is a challenging research field. We have demonstrated a real-time atomic force microscope by implementing a mechanical resonator type scanner called by "microscanner" The microscanner was designed to have a resonance frequency in the range of 5-10 kHz and an amplitude of 1-3 microm. The resonant vibration of the microscanner was served as a fast-scan directional motion, and an image acquisition rate of 30 frames/s with 256x256 pixels per frame was achieved. Time-varying sequential images of a poly(ethylene-oxide) sample were taken as a demonstration of potential for excellence in real-time imaging a moving nano-object.
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http://dx.doi.org/10.1063/1.2999579 | DOI Listing |
Langmuir
January 2025
Department of Physical Chemistry II, University of Bayreuth, Universitaetsstrasse 30, Bayreuth 95447, Germany.
Direct force measurements by atomic force microscopy (AFM) have become an indispensable analytical tool in the last decades. Force measurements have been widely used for adhesion measurements, often in combination with the colloidal probe technique. For the latter technique, a colloidal particle is attached to the end of an AFM cantilever, proving great flexibility in terms of colloid/surface interaction to be studied.
View Article and Find Full Text PDFBiomark Res
January 2025
Laboratory of Cellular Therapy, Department of Medical and Surgical Sciences for Children and Adults, University Hospital of Modena, Modena, 41124, Italy.
Emerging evidence highlights the key role of microRNA (miR)-21 in cell-to-cell communication and tumorigenesis. However, limited knowledge exists on the levels and clinical meaning of miR-21 in extracellular vesicles (EVs) of patients with breast cancer (BC). We assessed EV-derived miR-21 levels in one hundred women: 30 with early BC (EBC), 30 with metastatic BC on treatment progression (MBC), 30 cancer survivors on follow-up (FU) and 10 healthy donors (HD) as age- and body mass index (BMI)-matched controls.
View Article and Find Full Text PDFJ Chem Theory Comput
January 2025
Department of Physics, North Carolina State University, Raleigh, North Carolina 27695-8202, United States.
We present the theory, implementation, and benchmarking of a real-time time-dependent density functional theory (RT-TDDFT) module within the RMG code, designed to simulate the electronic response of molecular systems to external perturbations. Our method offers insights into nonequilibrium dynamics and excited states across a diverse range of systems, from small organic molecules to large metallic nanoparticles. Benchmarking results demonstrate excellent agreement with established TDDFT implementations and showcase the superior stability of our time integration algorithm, enabling long-term simulations with minimal energy drift.
View Article and Find Full Text PDFAdv Drug Deliv Rev
January 2025
Light sheet fluorescence microscopy (LSFM) has emerged as a transformative imaging technique in the study of drug delivery and embryonic development, offering high-resolution, real-time visualization with minimal phototoxicity. This review examines the application of LSFM in tracking drug pharmacokinetics, tissue-specific targeting, and drug efficacy during critical phases of embryonic development. Recent advancements in fluorescent labeling and machine learning integration have enabled more precise monitoring of drug release, distribution, and interaction with developing tissues.
View Article and Find Full Text PDFSci Rep
January 2025
College of Ocean Science and Engineering, Shandong University of Science and Technology, Qingdao, 266590, China.
Accurately predicting satellite clock deviation is crucial for improving real-time location accuracy in a GPS navigation system. Therefore, to ensure high levels of real-time positioning accuracy, it is essential to address the challenge of enhancing satellite clock deviation prediction when high-precision clock data is unavailable. Given the high frequency, sensitivity, and variability of space-borne GPS satellite atomic clocks, it is important to consider the periodic variations of satellite clock bias (SCB) in addition to the inherent properties of GPS satellite clocks such as frequency deviation, frequency drift, and frequency drift rate to improve SCB prediction accuracy and gain a better understanding of its characteristics.
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