A novel and sensitive multi-throughput localized surface plasmon resonance (MLSPR) biosensor was developed for the first time. Various gold nanorods with different aspect ratios were used to fabricate the optical sensor. Five kinds of gold nanorods with different aspect ratios were chosen to construct five throughputs of MLSPR. Various LSPR peaks imply that different acceptor-ligand pairs can be detected simultaneously in the wavelength range from 530 to 940nm. The biosensor immobilized on glass slides was applied to label-free detection between acceptor and ligand. The MLSPR-based optical biosensor can be used to detect three antigen-antibody pairs simultaneously. The biosensor proposed herein is easy to fabricate, and its operation procedure is convenient as labeling procedure is unnecessary.
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